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首页> 外文期刊>Journal of Clinical Microbiology >Multiplex PCR for Direct Detection of Shiga Toxigenic Escherichia coli Strains Producing the Novel Subtilase Cytotoxin
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Multiplex PCR for Direct Detection of Shiga Toxigenic Escherichia coli Strains Producing the Novel Subtilase Cytotoxin

机译:多重PCR技术直接检测产生新型枯草杆菌酶细胞毒素的志贺毒素产大肠杆菌

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We have recently described a novel AB5 subtilase cytotoxin produced by certain Shiga toxigenic Escherichia coli (STEC) strains. This potentially lethal toxin may contribute to severe gastrointestinal and systemic disease in humans. In this study we have developed a trivalent PCR assay for the detection of the novel toxin A subunit gene subA, as well as stx1 and stx2. The three primer pairs used in the assay do not interfere with each other and generate amplification products of 556, 180, and 255 bp, respectively. The assay can be used for determining the toxin genotype of STEC isolates, as well as for direct detection of toxin genes in primary fecal culture extracts.
机译:我们最近描述了某些志贺氏毒素大肠杆菌(STEC)菌株产生的新型AB 5 枯草杆菌蛋白酶。这种潜在的致命毒素可能会导致人类严重的胃肠道和全身疾病。在这项研究中,我们开发了一种三价PCR检测试剂盒,用于检测新型毒素A亚基基因 subA stx 1 和< em> stx 2 。测定中使用的三个引物对互不干扰,分别产生556、180和255 bp的扩增产物。该测定法可用于确定STEC分离物的毒素基因型,以及直接检测粪便培养物提取物中的毒素基因。

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