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首页> 外文期刊>Journal of Clinical Microbiology >Comparison of Illumigene, Simplexa, and AmpliVue Clostridium difficile Molecular Assays for Diagnosis of C. difficile Infection
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Comparison of Illumigene, Simplexa, and AmpliVue Clostridium difficile Molecular Assays for Diagnosis of C. difficile Infection

机译：Illumigene，Simplexa和AmpliVue艰难梭菌分子检测方法在艰难梭菌感染诊断中的比较

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We compared the performance of the Simplexa Universal Direct (Focus Diagnostics) and AmpliVue (Quidel Corporation) assays to that of the Illumigene assay (Meridian Bioscience, Inc.) for the diagnosis of Clostridium difficile infection. Two hundred deidentified remnant diarrheal stool specimens were tested by the Simplexa, AmpliVue, and Illumigene methods. Specimens with discrepant results among the three assays and a representative number of concordant specimens were further evaluated by toxigenic culture. The sensitivity and specificity were 98 and 100% and 96 and 100% for the Simplexa Universal Direct and AmpliVue assays, respectively. Both assays are easy to perform, with rapid turn-around-times, supporting their utility in the clinical laboratory as routine diagnostic platforms.
机译：我们比较了Simplexa Universal Direct（Focus Diagnostics）和AmpliVue（Quidel Corporation）检测与Illumigene检测（Meridian Bioscience，Inc.）检测诊断艰难梭菌感染的性能。通过Simplexa，AmpliVue和Illumigene方法测试了200个不确定的残留腹泻大便标本。通过产毒培养进一步评估了三种测定方法中结果不一致的标本和代表性标本的数量。对于Simplexa Universal Direct和AmpliVue检测，灵敏度和特异性分别为98％和100％，96和100％。两种检测方法都易于执行，并且具有快速的周转时间，从而支持了它们在临床实验室中作为常规诊断平台的用途。

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