...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Clinical Microbiology >Creation of an In-House Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Corynebacterineae Database Overcomes Difficulties in Identification of Nocardia farcinica Clinical Isolates
【24h】

Creation of an In-House Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Corynebacterineae Database Overcomes Difficulties in Identification of Nocardia farcinica Clinical Isolates

机译：建立室内基质辅助激光解吸电离-飞行时间质谱棒状杆菌数据库克服了诺卡氏菌临床分离株鉴定中的困难

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Nocardiosis is a rare disease that is caused by Gram-positive actinobacteria of the Nocardia genus and affects predominantly immunocompromised patients. In its disseminated form, it has a predilection for the central nervous system and is associated with high mortality rates. Therefore, prompt identification of the pathogen is critical. Matrix-assisted laser desorption ionization–time of flight (MALDI-TOF) mass spectrometry is a relatively novel technique used for identification of microorganisms. In this work, an upgraded MALDI-TOF Biotyper database containing Corynebacterineae representatives of strains deposited in the Polish Collection of Microorganisms was created and used for identification of the strain isolated from a nocardial brain abscess, mimicking a brain tumor, in an immunocompetent patient. Testing with the API Coryne system initially incorrectly identified Rhodococcus sp., while chemotaxonomic tests, especially mycolic acid analysis, enabled correct Nocardia identification only at the genus level. Subsequent sequence analysis of 16S rRNA and secA1 genes confirmed the identification. To improve the accuracy of the results, an in-house database was constructed using optimized parameters; with the use of the database, the strain was eventually identified as Nocardia farcinica. Clinical laboratories processing various clinical strains can upgrade a commercial database to improve and to accelerate the results obtained. This is especially important in the case of Nocardia, for which valid microbial diagnosis remains challenging; reference laboratories are often required to identify and to survey these rare actinobacteria.
机译：诺卡氏病是由诺卡氏菌属的革兰氏阳性放线菌引起的罕见疾病，主要影响免疫功能低下的患者。它以传播的形式偏向于中枢神经系统，并伴随着高死亡率。因此，迅速识别病原体至关重要。基质辅助激光解吸电离飞行时间质谱（MALDI-TOF）是用于鉴定微生物的一种相对较新的技术。在这项工作中，创建了一个升级的MALDI-TOF Biotyper数据库，该数据库包含保存在波兰微生物收藏中的菌株的棒状杆菌代表，并用于鉴定具有免疫能力的患者的从模仿心肌瘤的诺卡氏脑脓肿中分离出的菌株。使用API​​ Coryne系统进行的测试最初会错误地识别Rhodococcus sp。，而化学分类学测试（尤其是霉菌酸分析）只能在属水平上正确鉴定诺卡氏菌。随后对16S rRNA和 secA1 基因进行的序列分析证实了该鉴定。为了提高结果的准确性，使用优化的参数构建了一个内部数据库。利用该数据库，最终将该菌株鉴定为诺卡氏菌。处理各种临床菌株的临床实验室可以升级商业数据库，以改善和加速获得的结果。对于诺卡氏菌来说，这一点尤其重要，因为对于诺卡氏菌而言，有效的微生物诊断仍然很困难。通常需要参考实验室来鉴定和调查这些稀有的放线菌。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号