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首页> 外文期刊>Journal of Clinical Microbiology >Use of Noninvasive Markers To Detect Leishmania Infection in Asymptomatic Human Immunodeficiency Virus-Infected Patients
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Use of Noninvasive Markers To Detect Leishmania Infection in Asymptomatic Human Immunodeficiency Virus-Infected Patients

机译：使用无创标记物检测无症状人类免疫缺陷病毒感染患者的利什曼原虫感染

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Visceral leishmaniasis (VL) caused by Leishmania infantum is a common disease in human immunodeficiency virus (HIV)-infected people in the Mediterranean basin. However, most such cases are asymptomatic, and little information about the prevalence of these infections in HIV-infected individuals is available. The aim of this study was to assess the prevalence of subclinical infection and the relationship between several Leishmania infection markers by noninvasive methods in asymptomatic HIV-infected patients from Southern Spain. Ninety-two HIV-infected patients, who were consecutively attended at the participant hospitals in 2004, were invited to participate in this study. These patients were asymptomatic and without any history of cutaneous or visceral leishmaniasis. Leishmania kinetoplast DNA (kDNA) was amplified from peripheral blood samples from 28 (30.4%) of these HIV-infected subjects. Sera from three (3.5%) patients tested positive for Leishmania by an enzyme-linked immunoassay. Two patients (2.4%) showed a specific 16-kDa band by Western blotting. In contrast, none of the patients showed a positive agglutination of urine. The leishmanin skin test was positive for four (4.3%) patients. None of the patients with a PCR-positive result showed a positive reaction by enzyme-linked immunoassay or by specific bands in Western blotting or had a positive leishmanin skin test. In conclusion, L. infantum kDNA was detected in a large proportion of asymptomatic HIV-infected patients, although a demonstrable cellular or humoral immune response to this parasite was not shown. Conversely, Leishmania antigen in urine was not detected in these patients.
机译：由婴儿利什曼原虫引起的内脏利什曼病（VL）是地中海盆地人类免疫缺陷病毒（HIV）感染者的常见疾病。但是，大多数此类病例是无症状的，并且几乎没有关于这些感染在HIV感染者中患病率的信息。这项研究的目的是通过无创方法评估西班牙南部无症状HIV感染患者的亚临床感染患病率以及几种 Leishmania 感染标志物之间的关系。 2004年连续在参与医院就诊的92例HIV感染患者被邀请参加这项研究。这些患者无症状，无皮肤或内脏利什曼病史。从28名（30.4％）这些被HIV感染的受试者的外周血样本中扩增了 Leishmania 运动细胞DNA（kDNA）。通过酶联免疫法，三名（3.5％）患者的血清检测到利什曼原虫阳性。两名患者（2.4％）通过蛋白质印迹显示了一条特定的16kDa条带。相反，没有患者显示出尿液的正凝集。四名（4.3％）患者的利什曼宁皮肤试验阳性。 PCR阳性结果的患者均未通过酶联免疫测定或Western印迹法中的特定条带显示阳性反应，也未显示利什曼宁皮试阳性。总之， L。尽管没有显示出对这种寄生虫可证实的细胞或体液免疫反应，但仍在很大一部分无症状的HIV感染患者中检测到婴儿kDNA。相反，在这些患者中未检测到尿中的利什曼原虫抗原。

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