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首页> 外文期刊>Journal of Clinical Microbiology >Microbiological Evaluation of the New VITEK 2 Neisseria-Haemophilus Identification Card
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Microbiological Evaluation of the New VITEK 2 Neisseria-Haemophilus Identification Card

机译：新型VITEK 2奈瑟菌-嗜血杆菌识别卡的微生物学评估

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VITEK 2 is an automated identification system for diverse bacterial and fungal species. A new card (the Neisseria-Haemophilus [NH] card) for the identification of Neisseria spp., Haemophilus spp., and other fastidious gram-negative or gram-variable microorganisms has been developed, but its performance in a routine clinical laboratory has not yet been evaluated. In this study, a total of 188 bacterial strains belonging to the genera Actinobacillus, Campylobacter, Capnocytophaga, Cardiobacterium, Eikenella, Gardnerella, Haemophilus, Kingella, Moraxella, and Neisseria were investigated. The NH card was able to identify 171 strains (91%) correctly without the need for extra tests; one strain (0.5%) was misidentified, and five strains (2.7%) could not be classified. Eleven strains (5.8%) were identified with a low level of discrimination, and simple additional tests were required to increase the correct-identification rate to 96.8%. The results were available within 6 h. Based on these results, the new VITEK 2 NH card appears to be a good method for the identification of diverse groups of fastidious organisms, which would otherwise require testing with multiple systems. However, more work is needed to evaluate the performance of VITEK 2 with regard to Haemophilus, Actinobacillus, Cardiobacterium, Eikenella, and Kingella bacteria because of the insufficient number of strains tested in this study. Moreover, further reduction of the detection time would be desirable.
机译：VITEK 2是用于多种细菌和真菌物种的自动识别系统。用于识别 Neisseria spp。， Haemophilus 的新卡（ Neisseria -嗜血杆菌 [NH]卡）已经开发了Spp。和其他精制革兰氏阴性或革兰氏可变微生物，但是尚未评估其在常规临床实验室中的性能。在这项研究中，共有188个细菌菌株属于放线杆菌属，弯曲杆菌属，Capnocytophaga，卡门氏菌属，心型细菌， 艾肯氏菌，加德纳菌，嗜血杆菌， Kingella ，莫拉克氏菌和奈瑟氏球菌进行了调查。 NH卡能够正确识别171个菌株（91％），而无需进行额外的测试。一株（0.5％）被误认，五株（2.7％）无法分类。鉴定出11株（5.8％）的低歧视水平，并且需要进行简单的附加测试才能将正确鉴定率提高到96.8％。结果可在6小时内获得。基于这些结果，新的VITEK 2 NH卡似乎是识别不同类别的挑剔生物的好方法，否则将需要使用多个系统进行测试。但是，需要更多的工作来评估VITEK 2在嗜血杆菌，放线杆菌，心杆菌，艾肯氏菌方面的性能。 >和 Kingella 细菌，因为在这项研究中测试的菌株数量不足。此外，期望进一步减少检测时间。

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