Evaluation of Cytomegalovirus (CMV) DNA Quantification in Dried Blood Spots: Retrospective Study of CMV Congenital Infection

Christelle Vauloup-Fellous; Aurélie Ducroux; Vincent Couloigner; Sandrine Marlin; Olivier Picone; Julie Galimand; Natalie Loundon; Fran?oise Denoyelle; Liliane Grangeot-Keros; Marianne Leruez-Ville

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Evaluation of Cytomegalovirus (CMV) DNA Quantification in Dried Blood Spots: Retrospective Study of CMV Congenital Infection

机译：干血斑中巨细胞病毒（CMV）DNA定量的评估：CMV先天性感染的回顾性研究

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We compared two protocols for extracting DNA from dried blood spots for cytomegalovirus (CMV) DNA detection and quantification by real-time PCR. Both extraction methods were reliable for the retrospective diagnosis of CMV congenital infection. Quantification of CMV DNA was valuable after normalization of viral loads with albumin gene PCR amplification results.

机译：我们比较了从干血斑中提取DNA的两种方案，用于巨细胞病毒（CMV）DNA检测和实时PCR定量。两种提取方法均可用于CMV先天性感染的回顾性诊断。用白蛋白基因PCR扩增结果将病毒载量标准化后，CMV DNA的定量分析非常有价值。

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《Journal of Clinical Microbiology》 |2007年第11期|共3页
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Christelle Vauloup-Fellous; Aurélie Ducroux; Vincent Couloigner; Sandrine Marlin; Olivier Picone; Julie Galimand; Natalie Loundon; Fran?oise Denoyelle; Liliane Grangeot-Keros; Marianne Leruez-Ville;
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中图分类医学微生物学（病原细菌学、病原微生物学）;
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1. Evaluation of Different Cytomegalovirus (CMV) DNA PCR Protocols for Analysis of Dried Blood Spots from Consecutive Cases of Neonates with Congenital CMV Infections [J] . Oriane Soetens, Christelle Vauloup-Fellous, Ina Foulon, Journal of Clinical Microbiology . 2008,第3期

机译：评价不同的巨细胞病毒（CMV）DNA PCR方案以分析连续性先天性CMV感染病例的干血斑
2. CMV DNA detection in dried blood spots for diagnosing congenital CMV infection in Japan. [J] . Yamagishi Y, Miyagawa H, Wada K, Journal of Medical Virology . 2006,第7期

机译：在日本，干血斑中的CMV DNA检测可用于诊断先天性CMV感染。
3. Modification of CMV DNA detection from dried blood spots for diagnosing congenital CMV infection. [J] . Binda S, Caroppo S, Dido P, Journal of clinical virology: The official publication of the Pan American Society for Clinical Virology . 2004,第3期

机译：修改干血斑中CMV DNA检测以诊断先天性CMV感染。
4. Microfluidic device for rapid detection of cytomegalovirus (CMV) by sequence-specific hybridization of PCR-amplified CMV-DNA [C] . Briones, M.P., Yamashita, . 2006

机译：通过PCR扩增的CMV-DNA的序列特异性杂交快速检测巨细胞病毒（CMV）的微流体装置
5. Human cytomegalovirus infection in human umbilical vein endothelial cells; human cytomegalovirus infection in hematopoietic progenitor cells: Model of HCMV latency [D] . Zhuravskya, Tatiana. 1997

机译：人脐静脉内皮细胞中的人巨细胞病毒感染；造血祖细胞中人类巨细胞病毒感染：HCMV潜伏期模型
6. Evaluation of Cytomegalovirus (CMV) DNA Quantification in Dried Blood Spots: Retrospective Study of CMV Congenital Infection [O] . Christelle Vauloup-Fellous, Aurélie Ducroux, Vincent Couloigner, 2007

机译：干血斑中巨细胞病毒（CMV）DNA定量的评估：CMV先天性感染的回顾性研究
7. Evaluation of Cytomegalovirus (CMV) DNA Quantification in Dried Blood Spots: Retrospective Study of CMV Congenital Infection▿ [O] . Vauloup-Fellous, Christelle, Ducroux, Aurélie, Couloigner, Vincent, 2007

机译：干血斑中巨细胞病毒（CMV）DNA定量的评估：CMV先天性感染的回顾性研究▿

Evaluation of Cytomegalovirus (CMV) DNA Quantification in Dried Blood Spots: Retrospective Study of CMV Congenital Infection

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