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首页> 外文期刊>Journal of Clinical Microbiology >Identification of Mycobacterium Species and Mycobacterium tuberculosis Complex Resistance Determinants by Use of PCR-Electrospray Ionization Mass Spectrometry
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Identification of Mycobacterium Species and Mycobacterium tuberculosis Complex Resistance Determinants by Use of PCR-Electrospray Ionization Mass Spectrometry

机译：PCR-电喷雾电离质谱法鉴定分枝杆菌种类和结核分枝杆菌复杂的耐药性决定因素

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PCR coupled with electrospray ionization mass spectrometry (PCR–ESI-MS) is a novel technology that has recently been used to identify pathogens from clinical specimens or after culture within about 6 h. We evaluated the MDR-TB (multidrug-resistant tuberculosis) assay, which uses PCR–ESI-MS for detection and identification of Mycobacterium spp. and Mycobacterium tuberculosis complex (MTBC) resistance determinants from solid and broth Middlebrook culture media. The performance of the MDR-TB assay was compared to identification using nucleic acid hybridization probes and 16S rRNA gene sequencing for 68 MTBC and 97 nontuberculous mycobacterial (NTM) isolates grown on agar and 107 cultures grown in Bactec MGIT broth. MTBC resistance profiles from the MDR-TB assay were compared to results with the agar proportion method. The PCR–ESI-MS system correctly identified all MTBC isolates and 97.9% and 95.8% of the NTM isolates from characterized agar cultures and MGIT broth cultures to the species level, respectively. In comparison to the agar proportion method, the sensitivity and specificity for the detection of drug resistance using the MDR-TB assay were 100% and 92.3% for rifampin, 100% and 93.8% for isoniazid, 91.6% and 94.4% for ethambutol, and 100% and 100% for fluoroquinolones, respectively. The MDR-TB assay appears to be a rapid and accurate method for the simultaneous detection and identification of mycobacterial species and resistance determinants of MTBC from culture.
机译：PCR与电喷雾电离质谱（PCR-ESI-MS）结合是一项新技术，最近已被用于从临床标本或培养后约6小时内鉴定病原体。我们评估了MDR-TB（耐多药结核病）测定法，该测定法使用PCR-ESI-MS检测和鉴定分枝杆菌属。固体和肉汤Middlebrook培养基中的结核分枝杆菌复合物（MTBC）抗性决定因素。将MDR-TB分析的性能与使用核酸杂交探针和16S rRNA基因测序进行了比较，结果表明在琼脂上生长的68种MTBC和97种非结核分枝杆菌（NTM）分离物以及在Bactec MGIT肉汤中培养的107种培养物。将来自MDR-TB分析的MTBC耐药性与琼脂比例法的结果进行比较。 PCR-ESI-MS系统分别从特征​​琼脂培养物和MGIT肉汤培养物中正确鉴定出所有MTBC分离株以及97.9％和95.8％的NTM分离株，达到物种水平。与琼脂比例法相比，使用MDR-TB分析检测耐药性的敏感性和特异性分别为：利福平为100％和92.3％，异烟肼为100％和93.8％，乙胺丁醇为91.6％和94.4％，以及氟喹诺酮类分别为100％和100％。 MDR-TB分析似乎是一种快速准确的方法，可用于同时从培养物中检测和鉴定分枝杆菌菌种和MTBC的耐药性决定因素。

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