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首页> 外文期刊>Journal of Clinical Microbiology >Production of 2-Aminophenoxazin-3-one by Staphylococcus aureus Causes False-Positive Results in β-Galactosidase Assays
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Production of 2-Aminophenoxazin-3-one by Staphylococcus aureus Causes False-Positive Results in β-Galactosidase Assays

机译:金黄色葡萄球菌产生的2-氨基苯恶嗪-3-酮导致β-半乳糖苷酶测定的假阳性结果

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Staphylococcus aureus can be distinguished from similar coagulase-positive staphylococci by its absence of β-galactosidase activity. This is commonly tested using o-nitrophenyl-β-d-galactopyranoside (ONPG) as the substrate. Unexpectedly, 111 and 58 of 123 isolates displayed apparent β-galactosidase activity in the ONPG assay and on the Vitek 2 system, respectively. Compositional analysis showed that the yellow coloration of the positive ONPG assay resulted from production of 2-aminophenoxazin-3-one. Alternative β-galactosidase substrates like X-Gal (5-bromo-4-chloro-3-indolyl-β-d-galactopyranoside) should be used for testing staphylococci.
机译:金黄色葡萄球菌可以通过缺乏β-​​半乳糖苷酶活性而与相似的凝固酶阳性葡萄球菌区分开。通常使用 o -硝基苯基-β-d-吡喃半乳糖苷(ONPG)作为底物进行测试。出乎意料的是,123个分离株中的111个和58个在ONPG分析中和Vitek 2系统中分别显示出明显的β-半乳糖苷酶活性。组成分析表明,阳性ONPG分析呈黄色是由于产生了2-aminophenoxazin-3-one。应使用替代性的β-半乳糖苷酶底物(例如X-Gal)(5-溴-4-氯-3-吲哚基-β-d-吡喃半乳糖苷)来测试葡萄球菌。

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