Rapid Identification of Bacteria Directly from Positive Blood Cultures by Use of a Serum Separator Tube, Smudge Plate Preparation, and Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

Yan Chen; Vanessa Porter; Samira Mubareka; Leona Kotowich; Andrew E. Simor

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Rapid Identification of Bacteria Directly from Positive Blood Cultures by Use of a Serum Separator Tube, Smudge Plate Preparation, and Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

机译：通过使用血清分离管，污泥板制备和基质辅助激光解吸电离–飞行时间质谱直接从阳性血液培养物中快速鉴定细菌

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We analyzed the matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) of smudge plate growth for bacterial identification from 400 blood cultures. Ninety-seven percent of Gram-negative bacilli and 85% of Gram-positive organisms were correctly identified within 4 h; only eight isolates (2.0%) were misidentified. This method provided rapid and accurate microbial identification from positive blood cultures.

机译：我们分析了污泥板生长的基质辅助激光解吸电离-飞行时间质谱（MALDI-TOF MS），用于从400种血液培养物中鉴定细菌。在4 h内正确鉴定出了百分之九十七的革兰氏阴性杆菌和百分之八十五的革兰氏阳性菌。仅有8个隔离株（2.0％）被误认。此方法可从阳性血液培养物中快速，准确地鉴定微生物。

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《Journal of Clinical Microbiology》 |2015年第10期|共4页
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Yan Chen; Vanessa Porter; Samira Mubareka; Leona Kotowich; Andrew E. Simor;
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中图分类医学微生物学（病原细菌学、病原微生物学）;
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1. Impact of rapid microbial identification directly from positive blood cultures using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry on patient management [J] . MartinyD., DebaugniesF., GateffD., Clinical microbiology and infection: European Society of Clinical Microbiology and Infectious Diseases . 2013,第12期

机译：使用基质辅助激光解吸/电离飞行时间质谱直接从阳性血液培养物中快速鉴定微生物对患者管理的影响
2. Impact of rapid microbial identification directly from positive blood cultures using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry on patient management [J] . MartinyD., DebaugniesF., GateffD., Clinical microbiology and infection: European Society of Clinical Microbiology and Infectious Diseases . 2013,第12期

机译：使用基质辅助激光解吸/电离飞行时间质谱法直接从阳性血液培养的快速微生物鉴定的影响患者管理
3. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF) directly from positive blood culture flasks allows rapid identification of bloodstream infections in immunosuppressed hosts [J] . Egli A., Osthoff M., Goldenberger D., Transplant infectious disease: an official journal of the Transplantation Society . 2015,第3期

机译：直接从阳性培养瓶中进行基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF）可以快速鉴定免疫抑制宿主中的血流感染
4. Matrix-Assisted Laser Desorption Ionization/Time-of-Flight Mass Spectrometry as A Rapid and Sensitive Detection Method of Occult Blood in Feces [C] . Chen-I Wu, Pei-Chang Wu, Ching-Chou Tsai, ASMS Conference on Mass Spectrometry and Allied Topics . 2006

机译：基质辅助激光解吸电离/飞行时间质谱作为粪便中神经血液的快速和敏感的检测方法
5. Laser desorption/laser ionization time-of-flight mass spectrometry instrument design and investigation of the desorption and ionization mechanisms of matrix-assisted laser desorption/ionization. [D] . King, Richard Charles, III. 1994

机译：激光解吸/激光电离飞行时间质谱仪的设计以及基质辅助激光解吸/电离的解吸和电离机理研究。
6. Rapid Identification of Bacteria Directly from Positive Blood Cultures by Use of a Serum Separator Tube Smudge Plate Preparation and Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry [O] . Yan Chen, Vanessa Porter, Samira Mubareka, 2015

机译：通过使用血清分离管污泥板制备和基质辅助激光解吸电离–飞行时间质谱直接从阳性血液培养物中快速鉴定细菌
7. Impact of rapid microbial identification directly from positive blood cultures using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry on patient management. [O] . Martiny, D, Debaugnies, France, Gateff, D, 2013

机译：使用基质辅助激光解吸/电离飞行时间质谱直接从阳性血液培养物中快速鉴定微生物对患者管理的影响。

Rapid Identification of Bacteria Directly from Positive Blood Cultures by Use of a Serum Separator Tube, Smudge Plate Preparation, and Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

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