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首页> 外文期刊>Journal of Clinical Microbiology >A Novel Solid Medium for Culturing Mycobacterium tuberculosis Isolates from Clinical Specimens
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A Novel Solid Medium for Culturing Mycobacterium tuberculosis Isolates from Clinical Specimens

机译：从临床标本培养结核分枝杆菌的新型固体培养基。

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The laboratory diagnosis of tuberculosis usually relies on culture-based isolation of the causative Mycobacterium tuberculosis bacteria. We developed and evaluated the performance of MOD9, a new blood-free derivative of the MOD4 solid medium on which we previously reported for the isolation and culture of mycobacteria. First, inoculation of Lowenstein-Jensen medium with 21 M. tuberculosis isolates at 105, 103, and 10 CFU yielded colonies in 5.7 ± 1.5 days, 7.6 ± 0.8 days, and 10.8 ± 1.7 days versus 1.5 ± 0.4 days, 3.5 ± 0.6 days, and 4.9 ± 1 days in MOD9 (P < 0.05, Student's t test). Further, the time to detectable growth of M. tuberculosis was measured on MOD9 and Lowenstein-Jensen media after duplicate inoculation of 250 clinical specimens decontaminated with 0.7% chlorhexidine. The contamination rate was 1.6% (4/250) on MOD9 versus 4.4% (11/250) on Lowenstein-Jensen medium (P = 0.11, Fisher's exact test). Chlorhexidine-MOD9 yielded 38/250 (15.2%) isolates versus 32/250 (12.8%) isolates for the chlorhexidine-LJ (P = 0.5195, Fisher's exact test). All together, eight M. tuberculosis isolates were cultured solely from chlorhexidine-MOD9, and two M. tuberculosis isolates were cultured solely from chlorhexidine-LJ. The time to detection was 9.8 ± 3.9 (range, 5 to 18) days for chlorhexidine-MOD9 versus 17.4 ± 5.9 (range, 10 to 35) days for chlorhexidine-LJ (P < 0.05, Student's t test). These data indicate the superiority of the MOD9 medium over the standard LJ medium following chlorhexidine decontamination for the recovery of M. tuberculosis.
机译：结核病的实验室诊断通常依赖于病原性结核分枝杆菌的基于培养物的分离。我们开发并评估了MOD9的性能，MOD9是MOD4固体培养基的一种新的无血衍生物，我们之前曾在其上报道过该物质用于分离和培养分枝杆菌。首先，以5.7±1.5天，7.6±0.8天，10 5 ，10 3 和10 CFU接种21株结核分枝杆菌对Lowenstein-Jensen培养基进行接种，分别为10.8±1.7天和MOD9中的1.5±0.4天，3.5±0.6天和4.9±1天（ P <0.05，Student's t 测试）。此外，重复接种250份经0.7％洗必泰消毒的临床标本后，在MOD9和Lowenstein-Jensen培养基上测定了可检测到的结核分枝杆菌生长的时间。 MOD9的污染率为1.6％（4/250），而Lowenstein-Jensen培养基的污染率为4.4％（11/250）（ P = 0.11，Fisher精确检验）。洗必泰MOD9的分离株为38/250（15.2％），而洗必泰LJ的分离株为32/250（12.8％）（ P = 0.5195，Fisher精确检验）。总之，仅从洗必泰-MOD9培养了八个结核分枝杆菌分离株，而仅从洗必泰-LJ培养了两个结核分枝杆菌分离株。洗必泰-MOD9的检测时间为9.8±3.9天（5至18天），而洗必泰-LJ的检测时间为17.4±5.9（10至35天）（ P <0.05，学生的< em> t 测试）。这些数据表明，在洗必泰去污后，MOD9培养基优于标准LJ培养基对结核分枝杆菌的回收。

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