Preliminary Evaluation of the Research-Use-Only (RUO) iCubate iC-GPC Assay for Identification of Select Gram-Positive Bacteria and Their Resistance Determinants in Blood Culture Broths

Blake W. Buchan; Garrett C. Reymann; Paul A. Granato; Brenda R. Alkins; Patricia Jim; Stephen Young

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Preliminary Evaluation of the Research-Use-Only (RUO) iCubate iC-GPC Assay for Identification of Select Gram-Positive Bacteria and Their Resistance Determinants in Blood Culture Broths

机译：仅供研究（RUO）iCubate iC-GPC分析用于鉴定血液培养肉汤中所选革兰氏阳性细菌及其抗性决定因素的初步评估

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The iC-GPC assay (iCubate, Huntsville, AL) provides a molecular option for the rapid, on-demand analysis of positive blood cultures. A preliminary evaluation of the iC-GPC assay using 203 clinical or seeded specimens demonstrated a sensitivity of 93.8% to 100% and a specificity of 98.0% to 100% for the identification of five Gram-positive bacterial species (Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pneumoniae, Enterococcus faecalis, and Enterococcus faecium) and three associated genetic resistance determinants (mecA, vanA, and vanB) in positive blood culture broths.

机译：iC-GPC分析（iCubate，美国亨茨维尔，阿拉巴马州）为阳性血液培养物的按需快速分析提供了分子选择。使用203个临床或种子标本对iC-GPC分析进行的初步评估表明，对于鉴定5种革兰氏阳性细菌（金黄色葡萄球菌，表皮葡萄球菌，表皮葡萄球菌）的敏感性为93.8％至100％，特异性为98.0％至100％。阳性血液培养肉汤中的肺炎链球菌，粪肠球菌和粪肠球菌以及三个相关的遗传抗性决定因素（ mecA ， vanA 和 vanB ）。 展开▼

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《Journal of Clinical Microbiology》 |2015年第12期|共4页

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Blake W. Buchan; Garrett C. Reymann; Paul A. Granato; Brenda R. Alkins; Patricia Jim; Stephen Young;
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中图分类医学微生物学（病原细菌学、病原微生物学）;

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专利

1. Clinical Evaluation of the iCubate iC-GPC Assay for Detection of Gram-Positive Bacteria and Resistance Markers from Positive Blood Cultures [J] . Paul A. Granato, Melissa M. Unz, Raymond H. Widen, Journal of Clinical Microbiology . 2018,第9期

机译：iCubate iC-GPC检测从阳性血培养物中检测革兰氏阳性细菌和耐药标记的临床评估

2. Multiplex Identification of Gram-Positive Bacteria and Resistance Determinants Directly from Positive Blood Culture Broths: Evaluation of an Automated Microarray-Based Nucleic Acid Test [J] . Blake W. Buchan, Christine C. Ginocchio, Ryhana Manii, PLoS Medicine . 2013,第7期

机译：直接从阳性血培养液中直接鉴定革兰氏阳性细菌和抗性决定子的多重鉴定：基于自动微阵列的核酸测试的评估

3. Identification of Gram-Negative Bacteria and Genetic Resistance Determinants from Positive Blood Culture Broths by Use of the Verigene Gram-Negative Blood Culture Multiplex Microarray-Based Molecular Assay [J] . Nathan A. Ledeboer, Bert K. Lopansri, Neelam Dhiman, Journal of Clinical Microbiology . 2015,第8期

机译：使用基于Verigene革兰氏阴性血培养多重基因芯片的分子鉴定从阳性血培养液中鉴定革兰氏阴性细菌和遗传抗性决定因素

4. Antimicrobial use and resistance in selected zoonotic bacteria in aquaculture: preliminary findings of a survey of aquaculture-allied professionals [C] . Natasa Tusevljak, Andrijana Rajic, Lucie Dutil, FAO/AAHRI Expert Workshop on Improving Biosecurity through Prudent and Responsible Use of Veterinary Medicines in Aquatic Food Production . 2012

机译：水产养殖中所选人畜共患菌的抗菌药物使用和抗性：水产养殖专业人士调查的初步调查

5. The design, synthesis, and preliminary evaluation of novel antiviral photosensitizers for the purification of selected blood products. [D] . Aquila, Brian Michael. 1997

机译：新型抗病毒光敏剂的设计，合成和初步评估，用于纯化选定的血液制品。

6. Preliminary Evaluation of the Research-Use-Only (RUO) iCubate iC-GPC Assay for Identification of Select Gram-Positive Bacteria and Their Resistance Determinants in Blood Culture Broths [O] . Blake W. Buchan, Garrett C. Reymann, Paul A. Granato, 2015

机译：仅供研究（RUO）iCubate iC-GPC分析用于鉴定血液培养肉汤中所选革兰氏阳性细菌及其耐药性决定因素的初步评估

7. Multiplex identification of gram-positive bacteria and resistance determinants directly from positive blood culture broths: evaluation of an automated microarray-based nucleic acid test. [O] . Blake W Buchan, Christine C Ginocchio, Ryhana Manii, 2013

机译：直接从阳性血培养液中多重鉴定革兰氏阳性细菌和抗性决定簇：评估基于自动微阵列的核酸测试。

8. Class II Special Controls Guideline: Multiplex Nucleic Acid Assay for Identification of Microorganisms and Resistance Markers from Positive Blood Cultures Guideline for Industry. [R] . Sconc, K. 2015

机译：II类特殊控制指南：用于鉴定微生物和阳性血液培养抗性标记的多重核酸分析工业指南。

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2. 磁共振成像用于血液病患者铁过载评估的初步研究 [J] . 肖超 ,顾树程 ,徐燕军 . 中华血液学杂志 . 2015,第012期

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6. 卵泡液和胚胎培养液中MMP9的ELISA法测定及其应用于评估胚胎质量的初步探讨 [C] . 史艳彬 ,邵小光 . 中华医学会第六次全国生殖医学学术会议 . 2012

7. 卵泡液和胚胎培养液中MMP9的ELISA法测定及其应用于评估胚胎质量的初步探讨 [A] . 史艳彬 . 2007

1. 用于培养神经细胞的装置、用于培养神经细胞的方法、培养的神经细胞、用于分析和鉴定轴突束中蛋白质的方法、以及用于使用神经细胞的方法 [P] . 中国专利： CN109219655A . 2019-01-15

2. Culture Broth For Gram-Positive Bacteria [P] . 外国专利： KR20210123987A . 2021-10-14

机译：革兰氏阳性细菌的培养肉汤

3. Culture Broth For Gram-Positive Bacteria [P] . 外国专利： KR102165688B1 . 2020-10-14

机译：革兰氏阳性细菌培养液

4. Large scale prodn. of cooked blood broth - forr culture of e.g. haemophilic bacteria, involving proteolytic digestion of blood proteins [P] . 外国专利： DE4017489A1 . 1991-01-10

机译：大规模生产煮熟的肉汤-用于培养嗜血细菌，涉及血液蛋白的蛋白水解消化

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Preliminary Evaluation of the Research-Use-Only (RUO) iCubate iC-GPC Assay for Identification of Select Gram-Positive Bacteria and Their Resistance Determinants in Blood Culture Broths

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