...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Clinical Microbiology >Field Study of Dried Blood Spot Specimens for HIV-1 Drug Resistance Genotyping
【24h】

Field Study of Dried Blood Spot Specimens for HIV-1 Drug Resistance Genotyping

机译：HIV-1耐药基因分型干血斑标本的现场研究

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Dried blood spots (DBS) are an alternative specimen type for HIV drug resistance genotyping in resource-limited settings. Data relating to the impact of DBS storage and shipment conditions on genotyping efficiency under field conditions are limited. We compared the genotyping efficiencies and resistance profiles of DBS stored and shipped at different temperatures to those of plasma specimens collected in parallel from patients receiving antiretroviral therapy in Uganda. Plasma and four DBS cards from anti-coagulated venous blood and a fifth card from finger-prick blood were prepared from 103 HIV patients with a median viral load (VL) of 57,062 copies/ml (range, 1,081 to 2,964,191). DBS were stored at ambient temperature for 2 or 4 weeks or frozen at ?80°C and shipped from Uganda to the United States at ambient temperature or frozen on dry ice for genotyping using a broadly sensitive in-house method. Plasma (97.1%) and DBS (98.1%) stored and shipped frozen had similar genotyping efficiencies. DBS stored frozen (97.1%) or at ambient temperature for 2 weeks (93.2%) and shipped at ambient temperature also had similar genotyping efficiencies. Genotyping efficiency was reduced for DBS stored at ambient temperature for 4 weeks (89.3%, P = 0.03) or prepared from finger-prick blood and stored at ambient temperature for 2 weeks (77.7%, P < 0.001) compared to DBS prepared from venous blood and handled similarly. Resistance profiles were similar between plasma and DBS specimens. This report delineates the optimal DBS collection, storage, and shipping conditions and opens a new avenue for cost-saving ambient-temperature DBS specimen shipments for HIV drug resistance (HIVDR) surveillances in resource-limited settings.
机译：在资源有限的环境中，干血斑（DBS）是HIV耐药基因分型的另一种标本类型。在现场条件下，与DBS储存和运输条件对基因分型效率的影响有关的数据是有限的。我们比较了在不同温度下储存和运输的DBS的基因分型效率和抗性概况，与从乌干达接受抗逆转录病毒治疗的患者平行采集的血浆标本相比。从103名HIV患者中提取抗血浆静脉血的血浆和四张DBS卡，从手指刺血中提取第五张卡，中位病毒载量（VL）为57,062拷贝/ ml（范围1,081至2,964,191）。 DBS在室温下保存2或4周，或在80°C下冷冻，然后在室温下从乌干达运到美国，或在干冰上冷冻，以使用广泛敏感的内部方法进行基因分型。冷藏和运输的血浆（97.1％）和DBS（98.1％）具有相似的基因分型效率。冷冻（97.1％）或在环境温度下保存2周（93.2％）并在环境温度下运输的DBS也具有相似的基因分型效率。对于在室温下储存4周（89.3％， P = 0.03）或由手指刺血制备并在室温下储存2周的DBS（77.7％， P <0.001），与采用静脉血制备的DBS相比，处理方法相似。血浆和DBS标本之间的电阻曲线相似。该报告描述了最佳的DBS收集，存储和运输条件，并为节约资源的环境温度DBS标本运输开辟了一条新途径，以便在资源有限的环境中进行HIV耐药性（HIVDR）监测。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号