• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Clinical and Diagnostic Research >Comparative Evaluation of Four Phenotypic Tests for Detection of Metallo-?-Lactamase and Carbapenemase Production in Acinetobacter baumannii
【24h】

Comparative Evaluation of Four Phenotypic Tests for Detection of Metallo-?-Lactamase and Carbapenemase Production in Acinetobacter baumannii

机译：四种表型测试用于检测鲍曼不动杆菌中金属-β-内酰胺酶和碳青霉烯酶生产的比较评价

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Introduction: Acinetobacter baumannii is an emerging multi-drug resistant opportunistic pathogen that causes a variety of nosocomial infections. In recent years, carbapenem resistance in A.baumannii has increased due to Ambler class B Metallo ?-lactamases or class D OXA Carbapenemases.Objective: The present study was undertaken to detect and compare the various phenotypic methods for MBL production in nosocomial A.baumannii isolates. Materials and Methods: One hundred sixty eight A.baumannii isolates were subjected to disc diffusion assay. Imipenem resistant isolates were subjected to 4 different phenotypic tests. MBL screening was done by Imipenem-EDTA double disc synergy test, Imipenem-EDTA combined disc test, Modified Hodge test and MBL E-test.Results: Out of 168 A.baumannii isolates, 85 (50.59%) were imipenem resistant. Among these 85 isolates, 57 (67.05%) were MBL positive by DDST, 69 (81.18%) by CDT, 85 (100%) by MHT and all these 85 isolates were confirmed to be MBL positive by MBL E-test method.Conclusion: Combined disc test, Modified Hodge test & E-test are equally effective to detect MBL production. However, considering the cost constraints of E-test, simple MHT and CDT can be used. They are easy, economical and can be incorporated into routine testing in laboratories to monitor the emergence of MBLs in MDR A.baumannii.
机译：简介：鲍曼不动杆菌是一种新兴的多重耐药性机会病原体，可引起多种医院感染。近年来，由于Ambler B类金属β-内酰胺酶或D类OXA碳青霉烯酶，鲍曼不动杆菌对碳青霉烯的耐药性增加。隔离株。材料与方法：对168株鲍曼不动杆菌进行了圆盘扩散分析。对亚胺培南耐药的分离株进行了4种不同的表型测试。 MBI筛选通过亚胺培南-EDTA双盘协同试验，亚胺培南-EDTA联合盘试验，改良Hodge试验和MBL E检验进行。结果：在168株鲍曼不动杆菌中，有85株（50.59％）具有亚胺培南耐药性。在这85株菌株中，DDST的MBL阳性57株（67.05％），CDT的69株（81.18％），MHT的85株（100％），通过MBL E检验方法确认所有这85株菌株均为MBL阳性。 ：圆盘测试，改良式Hodge测试和E-测试相结合，对于检测MBL产生同样有效。但是，考虑到电子测试的成本约束，可以使用简单的MHT和CDT。它们简单，经济并且可以纳入实验室的常规测试中，以监测鲍氏不动杆菌中MBL的出现。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号