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首页> 外文期刊>Journal of Clinical and Diagnostic Research >Blood Agar for the Isolation of M. tuberculosis in Comparison with LJ Medium and BACTEC MGIT 960
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Blood Agar for the Isolation of M. tuberculosis in Comparison with LJ Medium and BACTEC MGIT 960

机译：与LJ培养基和BACTEC MGIT 960相比，用于分离结核分枝杆菌的血琼脂

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The evolution of laboratory diagnostic methods of Tuberculosis (TB) over the past few years are designed to achieve more rapid, less expensive, and accurate results. However, acid-fast staining and culture for Mycobacteria remains the core of any diagnostic algorithm for TB.Aim: To assess 7% Sheep Blood Agar (BA) for the primary isolation of Mycobacterium tuberculosis in comparison with the Lowenstein-Jensen (LJ) medium and BACTEC Mycobacterial Growth Inhibitor Tube (MGIT) 960.Materials and Methods: This is a prospective study and was carried over a period of seven months in State TB Demonstration and Training Centre Intermediate Reference Laboratory (STDC-IRL), Erragadda Hyderabad, India from March 2014 to September 2014. About 100 clinically suspected TB cases (95 pulmonary and 5 extra pulmonary) were selected and respective samples were subjected to Ziehl-Neelsen (ZN) staining. Twenty one samples were positive and 79 were negative for Acid-Fast Bacilli (AFB). The samples were processed and inoculated on BA slants, LJ medium and BACTEC MGIT 960 (Becton, Dickinson and Company) bottles. The cultures were confirmed by ZN staining and speciated by biochemical reactions. Further, validation of growth on BA was identified by Genotype MTBDR plus (Hains Lifescience, Nehren, Germany) to confirm the growth of M. tuberculosis.Results: Approximately 100 samples inoculated, 40 {38 M. tuberculosis and 2 Non tuberculous mycobacteria (NTM)} were isolated on MGIT 960, 37 (34 M. tuberculosis and 3 NTM) on BA and 32 (30 M. tuberculosis and 2 NTM) on LJ medium. The average isolation time of BA LJ and BACTEC MGIT 960 TB was 13.2 days, 26 days and 11.8 days. Nearly 37 blood culture positive samples tested by Genotype MTBDR plus, 34 (91.89%) were confirmed as Mycobacterium Tuberculosis Complex (MTBC). The contamination rate was 2% on BA, 6% on LJ and 12% on BACTEC MGIT 960.Conclusion: Primary isolation of M. tuberculosis was achieved 8 to 18 days after inoculation of clinical samples on BA. It requires no specific equipment and therefore, BA can be prepared, utilised and also have the advantage of shorter turnaround time.
机译：过去几年中，结核病（TB）实验室诊断方法的发展旨在实现更快，更便宜和更准确的结果。然而，分枝杆菌的耐酸染色和培养仍然是任何结核病诊断算法的核心。目标：与Lowenstein-Jensen（相比），评估7％的羊血琼脂（BA）用于结核分枝杆菌的初次分离（ LJ）培养基和BACTEC分枝杆菌生长抑制剂管（MGIT）960。材料与方法：这是一项前瞻性研究，在国家结核病示范与培训中心中级参考实验室（STDC-IRL）中进行了七个月的研究。 2014年3月至2014年9月，在印度海得拉巴的埃拉格达。选择了约100例临床疑似结核病例（95例肺结核和5例肺外结核），并对各个样本进行了Ziehl-Neelsen（ZN）染色。酸快速芽孢杆菌（AFB）的21个样品呈阳性，而79个阴性。将样品进行处理，然后接种到BA倾斜瓶，LJ培养基和BACTEC MGIT 960（Becton，Dickinson和Company）瓶上。通过ZN染色确认培养物，并通过生化反应确定培养物。此外，通过基因型MTBDR plus（Hains Lifescience，Nehren，Germany）鉴定了BA上的生长，以确认结核分枝杆菌的生长。结果：接种了约100个样品，其中40份{38结核分枝杆菌和2份非结核菌分枝杆菌（NTM）在MGIT 960上分离，在BA上37（34 M.结核和3 NTM）上分离，在LJ培养基上32（30 M.结核和2 NTM）上分离。 BA LJ和BACTEC MGIT 960 TB的平均隔离时间为13.2天，26天和11.8天。基因分型MTBDR plus检测的近37份血培养阳性样本被确认为结核分枝杆菌复合物（MTBC），占34（91.89％）。在BA上的污染率为2％，在LJ上为6％，在BACTEC MGIT 960上为12％。它不需要特定的设备，因此可以制备，使用BA，并且还具有缩短周转时间的优势。

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