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首页> 外文期刊>Journal of Clinical and Diagnostic Research >Biosynthesis, Characterization and Antibacterial Efficacy of Silver Nanoparticles Derived from Endophytic Fungi against P. gingivalis ZC92-ZC96
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Biosynthesis, Characterization and Antibacterial Efficacy of Silver Nanoparticles Derived from Endophytic Fungi against P. gingivalis ZC92-ZC96

机译：内生真菌衍生的银纳米颗粒对牙龈卟啉单胞菌ZC92-ZC96的生物合成，表征和抑菌作用

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Introduction: Microbial resistance to existing antimicrobial agents in periodontal therapy is a growing problem. Therefore, there is a need for development of new antimicrobial agents.Aim: To biosynthesize and characterize Silver Nanoparticles (AgNPs) using endophytic fungi and to evaluate the antibacterial efficacy against P. gingivalis.Materials and Methods: Cut leaf segments of Withania Somnifera (Ashwagandha) were used to isolate the fungi. Fresh cultures of fungi were inoculated in Erlenmeyer flask of 100 ml Malt Glucose Yeast Peptone (MGYP) broth and incubated at 29oC for 72 hours for the biomass to grow. Biomass was filtered and cell free fungal filtrate was used further. Biosynthesized AgNPs were characterized by visual observation, Ultraviolet-Visible (UV-Vis) spectrophotometer, Transmission Electron Microscopy (TEM), Selected Area Electron Diffraction Analysis (SAED) and Fourier Transform Infrared Spectroscopy (FTIR). Antibacterial efficacy was evaluated by agar diffusion method measuring the zone of inhibition. The study groups included different concentrations of AgNPs: A (20 μl), B (40 μl), C (60 μl), D (80 μl) and E (100 μl) of AgNPs, F (0.2% CHX), G (2% CHX), H (Ampicillin) and I (sterile distilled water). The data collected for inhibition zones were statistically analysed using One-way Anova followed by Tukey post-hoc multiple comparison tests.Results: The fungi were identified as Fusarium semitectum. Characterization studies showed the colour change from colourless to reddish brown; U-V spectrum showed peak 420 nm, TEM revealed the particles spherical in shape and 10-20 nm in size. FTIR analysis revealed the presence of functional groups. AgNPs 80 μl and 100 μl showed mean zone of inhibition 17.33 and 18 mm against P. gingivalis. CHX (0.2%) 17.85 and CHX (2%) 19.97 mm, Ampicillin 20.5 mm and no zone for sterile distilled water.Conclusion: Biosynthesized AgNPs showed efficient antibacterial efficacy against P. gingivalis hence, creates a new horizon in periodontal therapy.
机译：简介：牙周治疗中微生物对现有抗菌剂的耐药性是一个日益严重的问题。因此，有必要开发新的抗菌剂。目的：利用内生真菌生物合成和表征银纳米颗粒（AgNPs），并评估其对牙龈卟啉单胞菌的抗菌功效。 ）用于分离真菌。将新鲜的真菌培养物接种在100 ml麦芽葡萄糖酵母蛋白ept（MGYP）肉汤的锥形瓶中，并在29oC下孵育72小时，以使生物量生长。过滤生物质，并进一步使用无细胞的真菌滤液。通过视觉观察，紫外可见（UV-Vis）分光光度计，透射电子显微镜（TEM），选择区域电子衍射分析（SAED）和傅里叶变换红外光谱（FTIR）对生物合成的AgNP进行表征。通过琼脂扩散法测量抑制区域来评价抗菌效力。研究组包括不同浓度的AgNP：A（20μl），B（40μl），C（60μl），D（80μl）和E（100μl）AgNP，F（0.2％CHX），G（ 2％CHX），H（氨苄青霉素）和I（无菌蒸馏水）。采用单向方差分析和Tukey事后多重比较试验对抑制区收集的数据进行统计分析。结果：真菌被鉴定为半镰刀菌。表征研究表明，颜色从无色变为红棕色。 U-V光谱显示峰420nm，TEM显示颗粒为球形，尺寸为10-20nm。 FTIR分析表明存在官能团。 AgNP 80μl和100μl对齿龈假单胞菌的平均抑制区分别为17.33和18 mm。 CHX（0.2％）17.85 mm和CHX（2％）19.97 mm，氨苄青霉素20.5 mm，无无菌蒸馏水区域。

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