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首页> 外文期刊>Journal of bacteriology >Biosynthesis and Cellular Distribution of the Two Superoxide Dismutases of Dactylium dendroides
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Biosynthesis and Cellular Distribution of the Two Superoxide Dismutases of Dactylium dendroides

机译：Dactylium dendroides的两个超氧化物歧化酶的生物合成和细胞分布。

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The synthesis and subcellular localization of the two superoxide dismutases of Dactylium dendroides were studied in relation to changes in copper and manganese availability. Cultures grew normally at all medium copper concentrations used (10 nM to 1 mM). In the presence of high (10 μM) copper, manganese was poorly absorbed in comparison to the other metals in the medium. However, cells grown at 10 nM copper exhibited a 3.5-fold increase in manganese content, while the concentration of the other metals remained constant. Cultures grown at 10 nM copper or more had 80% Cu/Zn enzyme and 20% mangani enzyme; the former was entirely in the cytosol, and the latter was mitochondrial. Removal of copper from the medium resulted in decreased Cu/Zn superoxide dismutase synthesis with a concomitant increase in the mangani enzyme such that total cellular superoxide dismutase activity remained constant. The mangani enzyme in excess of the 20% was present in the non-mitochondrial fraction. The mitochondria, therefore, show no variability with respect to superoxide dismutase content, whereas the soluble fraction varies from 100 to 13% Cu/Zn superoxide dismutase. Copper-starved cells that were synthesizing predominantly mangani superoxide dismutase could be switched over to mostly Cu/Zn superoxide dismutase synthesis by supplementing the medium with copper during growth. Immunoprecipitation experiments suggest that the decrease in Cu/Zn activity at low copper concentration is a result of decreased synthesis of that protein rather than the production of an inactive apoprotein.
机译：研究了铜金属锰（Dactylium dendroides）的两种超氧化物歧化酶的合成和亚细胞定位与铜和锰利用率变化的关系。在所有使用的中等铜浓度（10 nM至1 mM）下，培养物均可正常生长。与介质中的其他金属相比，在存在高（10μM）铜的情况下，锰吸收不良。但是，以10 nM铜生长的细胞显示锰含量增加了3.5倍，而其他金属的浓度保持恒定。在10 nM铜或更多的铜上生长的培养物含有80％的Cu / Zn酶和20％的Mangani酶。前者完全在细胞质中，而后者是线粒体。从培养基中去除铜导致减少的Cu / Zn超氧化物歧化酶合成，并伴随增加mangani酶，使得总细胞超氧化物歧化酶活性保持恒定。非线粒体部分中存在超过20％的mangani酶。因此，线粒体在超氧化物歧化酶含量方面没有变化，而可溶性部分在100％至13％的Cu / Zn超氧化物歧化酶之间变化。通过在生长过程中向培养基补充铜，主要合成曼格尼超氧化物歧化酶的铜饥饿的细胞可以切换为大部分Cu / Zn超氧化物歧化酶的合成。免疫沉淀实验表明，低铜浓度下Cu / Zn活性的降低是该蛋白质合成减少的结果，而不是无活性载脂蛋白的产生。

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