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首页> 外文期刊>Journal of bacteriology >Isolation and characterization of amber mutations in the lexA gene of Escherichia coli K-12.
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Isolation and characterization of amber mutations in the lexA gene of Escherichia coli K-12.

机译：大肠杆菌K-12的lexA基因中琥珀色突变的分离和鉴定。

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We describe the isolation and characterization of amber mutations in the lexA gene of Escherichia coli K-12. These mutations, designated spr(Am), were isolated and characterized in a lexA tif sfi genetic background. They abolished the sensitivity of the strain to UV light and resulted in high rates of synthesis of recA protein. Phage lambda+ failed to lysogenize the strains as observed with similar strains carrying non-amber spr mutations described previously, thereby indicating a constitutive expression of the phage induction pathway. Introduction of an amber suppressor mutation into a strain bearing the spr(Am) mutation restored expression of the LexA mutant phenotype. We conclude that spr mutations either inactivate or prevent synthesis of the lexA gene product and that loss of this product results in constitutive expression of the E. coli induction system in the tif sfi genetic background.
机译：我们描述了大肠杆菌K-12的lexA基因中琥珀突变的分离和鉴定。这些突变称为spr（Am），在lexA tif sfi遗传背景中进行了分离和表征。他们取消了菌株对紫外线的敏感性，并导致了recA蛋白的高合成率。如先前所述的携带非琥珀色spr突变的类似菌株所观察到的，噬菌体lambda +未能裂解菌株，从而表明噬菌体诱导途径的组成型表达。将琥珀色抑制突变引入带有spr（Am）突变的菌株中可恢复LexA突变表型的表达。我们得出的结论是，spr突变会失活或阻止lexA基因产物的合成，并且该产物的缺失会导致tif sfi遗传背景下大肠杆菌诱导系统的组成型表达。

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