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首页> 外文期刊>Journal of bacteriology >Precursor-product relationship of intracellular and extracellular lipoteichoic acids of Streptococcus faecium.
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Precursor-product relationship of intracellular and extracellular lipoteichoic acids of Streptococcus faecium.

机译：粪链球菌细胞内和细胞外脂蛋白酸的前体产物关系。

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摘要

Exponential biosynthesis and excretion of lipoteichoic acid (LTA) during the exponential phase of growth, and continued synthesis and excretion during valine starvation of Streptococcus faecium (S. faecalis ATCC 9790), were shown. During exponential growth, extracellular LTA (LTAx) accounted for approximately 13% of the total LTA in cultures, whereas during valine starvation, this percentage increased to approximately 60% within 4 h. LTAx was present in a low-molecular-weight, apparently deacylated form, whereas intracellular (LTAi) was present primarily in an apparently high-molecular-weight, acylated and micellar form. Experiments utilizing chases of either fully equilibrated or short pulses of [14C]- or [3H]glycerol were used to demonstrate that LTAx was derived directly from LTAi.
机译：显示了在指数增长阶段的脂蛋白磷酸（LTA）的指数生物合成和排泄，以及粪便链球菌（S. faecalis ATCC 9790）的缬氨酸饥饿期间的持续合成和排泄。在指数生长期间，细胞外LTA（LTAx）约占培养物中总LTA的13％，而在缬氨酸饥饿期间，该百分比在4小时内增加到大约60％。 LTAx以低分子量，明显脱酰基的形式存在，而细胞内（LTAi）主要以明显高分子量，酰化和胶束的形式存在。利用利用完全平衡或短脉冲的[14C]-或[3H]甘油的追赶进行的实验来证明LTAx直接来自LTAi。

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