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首页> 外文期刊>Journal of bacteriology >Tight-binding repressors of the lac operon: selection system and in vitro analysis.
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Tight-binding repressors of the lac operon: selection system and in vitro analysis.

机译：lac操纵子的紧密结合阻遏物：选择系统和体外分析。

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摘要

The isolation and characterization of altered repressors of the lac operon which have an increased affinity for an operator should give useful clues about the molecular basis for the very tight and specific interaction between repressor and operator. A selection system has been devised which allows the isolation of such repressor mutants. This system selects for mutant repressors which can overcome lac operator-constitutive (Oc) mutations. By using in vivo assays, 24 candidates were obtained which, compared with wild type, have an increased trans effect of their repressor on one or several Oc operators. Three of these candidates have been investigated in vitro; the affinity of their repressor for inducer was unchanged, whereas the affinity for wild-type operator was increased 15-, 86-, and 262-fold, respectively.
机译：对操纵子具有更高亲和力的lac操纵子改变的阻遏物的分离和表征，应该为阻抑物和操纵基因之间非常紧密而特异的相互作用的分子基础提供有用的线索。已经设计了选择系统，其允许分离这种阻遏物突变体。该系统选择可以克服lac操纵子组成型（Oc）突变的突变阻遏物。通过体内试验，获得了24种候选物，与野生型相比，它们的阻遏物对一种或几种Oc操纵基因的反式作用增强。这些候选物中的三个已经在体外进行了研究。它们的阻遏物对诱导物的亲和力没有改变，而对野生型操纵子的亲和力则分别增加了15倍，86倍和262倍。

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