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首页> 外文期刊>Journal of bacteriology >Outer membrane proteins of Escherichia coli. V. Evidence that protein 1 and bacteriophage-directed protein 2 are different polypeptides.
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Outer membrane proteins of Escherichia coli. V. Evidence that protein 1 and bacteriophage-directed protein 2 are different polypeptides.

机译：大肠杆菌的外膜蛋白。 V.蛋白质1和噬菌体指导的蛋白质2是不同多肽的证据。

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摘要

Protein 1 from the outer membrane of Escherichia coli K-12 and protein 2 from a phage PA-2 lysogen of the same strain were isolated by differential sodium dodecyl sulfate extraction and purified by ion-exchange and gel filtration chromatography. Rabbit antisera were prepared against these proteins and showed no cross-reaction between proteins 1 and 2. The proteins have the same N-terminal amino acid but show small yet significant differences in amino acid composition. The proteins were cleaved with cyanogenbromide in solvents containing both formic acid and trifluoroacetic acid. By comparing the cleavage in these solvents, it was established that protein 1 yielded 5 cyanogen bromide peptides, and the sum of the molecular weights of these was equivalent to the molecular weight of the uncleaved protein. Protein 2 yielded 4 cyanogen bromide peptides, none of which was identical to those of protein 1, and the sum of these peptides was also equivalent to the apparent molecular weight of the uncleaved protein. Significant differences were also observed when tryptic peptides from the two proteins were compared. These results indicate that protein 1 and the phage-directed protein 2 are distinct, different, and apparently homogeneous proteins.
机译：通过差异十二烷基硫酸钠萃取分离来自大肠杆菌K-12外膜的蛋白质1和来自同一菌株的噬菌体PA-2溶原菌的蛋白质2，并通过离子交换和凝胶过滤色谱法纯化。制备了针对这些蛋白质的兔抗血清，它们在蛋白质1和2之间没有交叉反应。蛋白质具有相同的N端氨基酸，但氨基酸组成上显示出微小但显着的差异。在含有甲酸和三氟乙酸的溶剂中，用溴化氰裂解蛋白质。通过比较在这些溶剂中的裂解，可以确定蛋白质1产生了5个溴化氰肽，并且它们的分子量之和等于未裂解的蛋白质的分子量。蛋白质2产生4个溴化氰肽，这些肽都不与蛋白质1的肽相同，这些肽的总和也等于未切割蛋白质的表观分子量。比较来自两种蛋白质的胰蛋白酶肽时，也观察到了显着差异。这些结果表明蛋白1和噬菌体定向蛋白2是不同的，不同的，并且显然是同质的蛋白。

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