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首页> 外文期刊>Journal of bacteriology >Bacterial polysaccharide which binds Rhizobium trifolii to clover root hairs.
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Bacterial polysaccharide which binds Rhizobium trifolii to clover root hairs.

机译：将三叶根瘤菌与三叶草根毛结合的细菌多糖。

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摘要

Immunofluorescence, quantitative immunoprecipitation, and inhibition of bacterial agglutination and passive hemagglutination indicate that cross-reactive antigenic determinants are present on the surface of Rhizobium trifolii and clover roots. These determinants are immunochemically unique to this Rhizobium-legume cross-inoculation group. The multivalent lectin trifoliin and antibody to the clover root antigenic determinants bind competitively to two acidic heteropolysaccharides isolated from capsular material of R. Trifolii 0403. The major polysaccharide is an antigen which lacks heptose, 2-keto-3-deoxyoctulosonic acid, and endotoxic lipid A. The minor polysaccharide in the capsular material of R. Trifolii 0403 contains the same antigen in addition to heptose, 2-keto-3-deoxyoctonate, and lipid A. The acidic polysaccharides of two strains of R. trifolii share the clover r-ot cross-reactive antigenic determinant despite other differences in their carbohydrate composition. Studies with monovalent antigen-binding fragments of anti-clover root antibody and Azotobacter vinelandii hybrid transformants carrying the unique antigenic determinant suggest that these polysaccharides bind R. trifolii to the clover root hair tips which contain trifoliin.
机译：免疫荧光，定量免疫沉淀以及对细菌凝集和被动血凝的抑制作用表明三叶草根和三叶草根的表面存在交叉反应性抗原决定簇。这些决定簇在免疫化学上是该根瘤菌-豆科植物交叉接种组唯一的。三叶草根抗原决定簇的多价凝集素三叶草素和抗体与从三叶草0403荚膜材料中分离的两个酸性杂多糖竞争性结合。主要的多糖是缺乏庚糖，2-酮基-3-脱氧八聚核糖核酸和内毒素脂质的抗原。 A. R. Trifolii 0403的荚膜材料中的次要多糖除含有庚糖，2-酮-3-脱氧辛酸和脂质A外，还包含相同的抗原。两株R. Trifolii的酸性多糖均具有三叶草r-尽管它们的碳水化合物组成存在其他差异，但仍具有交叉反应性抗原决定簇。用抗三叶草根抗体的单价抗原结合片段和带有独特抗原决定簇的葡萄固氮杂种杂种转化体的研究表明，这些多糖将三叶利福氏菌结合到含有三叶草素的三叶草根毛尖上。

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