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首页> 外文期刊>Journal of bacteriology >Electron microscopy of the rodlet layer of Neurospora crassa conidia.
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Electron microscopy of the rodlet layer of Neurospora crassa conidia.

机译:景天孢子孢子子粒层的电子显微镜观察。

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摘要

Neurospora crassa macroconidia possess a regularly arranged layer of small fibers (rodlets) near the spore surface. The structure and location of this layer were studied by making surface replicas, by negative staining, by freeze-fracturing and deep-etching, and by thin sectioning. When conidia were shaken vigorously in water, the layer fragmented and became separated from the surface in sheets. Negative staining of such sheets showed that the individual rodlets have a hollow central core. When conidia were shaken gently in water or fixative, large fragments of the rodlet layer often remained on the conidial surface. The fragments tended to fold back on each other such that multiple layers were sometimes seen in thin sections. It is concluded that in dry conidia the rodlets are located on the extreme outside of the spore where they form a monolayer with only occasional regions of overlap.
机译:景天孢子菌的大分生孢子在孢子表面附近有规则排列的小纤维层(小杆)。通过制作表面复制品,负片染色,冷冻断裂和深蚀刻以及薄切片研究了该层的结构和位置。当分生孢子在水中剧烈摇动时,该层会碎裂并与表面成片状分离。这些薄片的阴性染色表明,单个小棒具有空心的中心核。将分生孢子在水中或固定剂中轻轻摇动时,通常在分生孢子表面上残留大片的小杆状层。碎片趋于彼此折回,因此有时会在薄切片中看到多层。结论是,在干分生孢子中,小棒位于孢子的最外层,在那里它们形成单层,只有偶尔的重叠区域。

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