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首页> 外文期刊>Journal of bacteriology >Identification of the purC gene product of Escherichia coli.
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Identification of the purC gene product of Escherichia coli.

机译:大肠杆菌purC基因产物的鉴定。

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The purC region of the Escherichia coli chromosome was isolated from in vivo-derived lambda transducing bacteriophages and cloned in high-copy-number plasmids. The product of the purC gene, phosphoribosylaminoimidazolesuccinocarboxamide synthetase, was identified as a protein with an Mr of ca. 27,000. The level of the protein is increased by more than 60-fold in strains carrying the gene on a high-copy-number plasmid. Purine addition represses the enzyme level in both plasmid- and non-plasmid-containing strains.
机译:从体内衍生的λ转导噬菌体中分离出大肠杆菌染色体的purC区,并克隆到高拷贝数质粒中。 purC基因的产物,磷酸核糖基氨基咪唑琥珀酰羧酰胺合成酶,被鉴定为Mr为约3的蛋白质。 27,000。在高拷贝数质粒上携带该基因的菌株中,蛋白质的水平增加了60倍以上。嘌呤的添加抑制含质粒和不含质粒的菌株中的酶水平。

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