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首页> 外文期刊>Journal of bacteriology >Physiological properties of cold-sensitive suppressor mutations of a temperature-sensitive dnaZ mutant of Escherichia coli.
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Physiological properties of cold-sensitive suppressor mutations of a temperature-sensitive dnaZ mutant of Escherichia coli.

机译：大肠杆菌对温度敏感的dnaZ突变体的冷敏感抑制子突变的生理特性。

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Suppressors of a temperature-sensitive dnaZ polymerization mutant of Escherichia coli have been identified by selecting temperature-insensitive revertants. Those suppressed strains which concomitantly became cold sensitive were chosen for further study. Intragenic suppressor mutations, which caused cold-sensitive defects in DNA polymerization, were located in dnaZ by transduction with lambda dnaZ+ phages. Extragenic suppressor mutations were mapped within the initiation gene dnaA. These suppressor-containing strains were defective in initiation at low temperature as determined by measurements of DNA synthesis in vivo and in toluene-treated cells. The occurrence of suppressor mutations of dnaZ(Ts) within the dnaA gene is considered evidence that the dnaA and dnaZ products interact in vivo. A second indication of a dnaA-dnaZ protein-protein interaction was provided by the observation that the introduction of additional copies of the dnaZ+ gene into a strain carrying the dnaA suppressor mutation was lethal [whether the strain was dnaZ+ or dnaZ(Ts)].
机译：大肠杆菌的温度敏感的dnaZ聚合突变体的抑制剂已通过选择对温度不敏感的回复株来鉴定。选择那些伴随变冷的抑制菌株进行进一步研究。导致DNA聚合反应中冷敏感缺陷的基因内抑制子突变通过λdnaZ +噬菌体的转导而位于dnaZ中。外源抑制突变定位在起始基因dnaA内。这些含抑制因子的菌株在低温下的启动有缺陷，这是通过体内和甲苯处理的细胞中DNA合成的测量确定的。 dnaA基因内dnaZ（Ts）抑制突变的发生被认为是dnaA和dnaZ产物在体内相互作用的证据。 dnaA-dnaZ蛋白相互作用的第二个迹象是观察到，将额外拷贝的dnaZ +基因引入携带dnaA抑制子突变的菌株是致命的[该菌株是dnaZ +还是dnaZ（Ts）]。

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