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首页> 外文期刊>Journal of bacteriology >Sequence analysis of the major outer membrane protein gene from Chlamydia trachomatis serovar L2.
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Sequence analysis of the major outer membrane protein gene from Chlamydia trachomatis serovar L2.

机译：沙眼衣原体血清型L2主要外膜蛋白基因的序列分析。

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The structural gene for the major outer membrane protein (MOMP) from Chlamydia trachomatis was cloned and sequenced. A lambda gt11 recombinant (lambda gt11/L2/33) that contains a portion of the MOMP coding sequence was used to probe a lambda 1059 library constructed from DNA obtained from C. trachomatis serovar L2. Selected lambda 1059 recombinants were mapped with endonuclease restriction enzymes. The MOMP gene was mapped to the 5' end of a BamHI fragment of approximately 9 kilobases. Contiguous endonuclease restriction fragments identified within this region permitted the selection of specific fragments for subcloning and DNA sequencing. The MOMP gene consisted of a 1,182-base-pair open reading frame that encoded 394 amino acids and ended with three stop codons. The known amino-terminal amino acid was preceded by 22 amino acids whose sequence was compatible with a leader or signal sequence. The primary structure of MOMP determined from the translated DNA sequence demonstrated nine cysteine residues and a remarkably homogeneous distribution of charged and hydrophobic residues.
机译：克隆并测序了沙眼衣原体主要外膜蛋白（MOMP）的结构基因。包含部分MOMP编码序列的λgt11重组体（λgt11 / L2 / 33）用于探测由从沙眼衣原体L2获得的DNA构建的λ1059文库。选定的λ1059重组体与核酸内切酶限制酶作图。 MOMP基因被定位到大约9个碱基的BamHI片段的5'端。在该区域内鉴定的连续的核酸内切酶限制性片段允许选择用于亚克隆和DNA测序的特定片段。 MOMP基因由一个1,182个碱基对的开放阅读框组成，该阅读框编码394个氨基酸，并以三个终止密码子结尾。已知的氨基末端氨基酸之前是22个氨基酸，其序列与前导序列或信号序列相容。由翻译的DNA序列确定的MOMP的一级结构显示出9个半胱氨酸残基以及带电和疏水残基的显着均匀分布。

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