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首页> 外文期刊>Journal of bacteriology >Are growth rates of Escherichia coli in batch cultures limited by respiration?
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Are growth rates of Escherichia coli in batch cultures limited by respiration?

机译：分批培养中大肠杆菌的生长速率是否受到呼吸的限制？

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Batch cultures of Escherichia coli were grown in minimal media supplemented with various carbon sources which supported growth at specific growth rates from 0.2 to 1.3/h. The respiration rates of the cultures were measured continuously. With few exceptions, the specific rate of oxygen consumption was about 20 mmol of O2/h per g (dry weight), suggesting that the respiratory capacity was limited at this value. The adenosine triphosphate (ATP) required for the production of cell material from the different carbon sources was calculated on the basis of known ATP requirements in the biochemical pathways and routes of macromolecular synthesis. The calculated ATP requirements, together with the measured growth rates and growth yields on the different carbon sources, were used to calculate the rate of ATP synthesis by oxidative phosphorylation. This rate was closely related to the respiration rate. We suggest that aerobic growth of E. coli in batch cultures is limited by the rate of respiration and the concomitant rate of ATP generation through oxidative phosphorylation.
机译：大肠杆菌的分批培养物在添加了多种碳源的基本培养基中生长，这些碳源支持以0.2至1.3 / h的特定生长速率生长。连续测量培养物的呼吸速率。除极少数例外，耗氧量的具体比率为每g（干重）约20 mmol O2 / h，这表明呼吸能力受到限制。从不同碳源生产细胞材料所需的三磷酸腺苷（ATP）是根据大分子合成的生化途径和途径中已知的ATP要求计算得出的。计算出的ATP需求，以及在不同碳源上测得的生长速率和生长产量，可用于通过氧化磷酸化来计算ATP的合成速率。该速率与呼吸速率密切相关。我们建议分批培养中大肠杆菌的有氧生长受到呼吸速率和通过氧化磷酸化产生的ATP伴随速率的限制。

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