...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of bacteriology >Glutamic acid codon suppressors derived from a unique species of glycine transfer ribonucleic acid.
【24h】

Glutamic acid codon suppressors derived from a unique species of glycine transfer ribonucleic acid.

机译：谷氨酸密码子抑制剂衍生自独特的甘氨酸转移核糖核酸。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In this paper we describe the successful isolation of glyT-derived GAA suppressors. A glyT+ strain containing glyV55, the gene for a GGA/G-reading, methane sulfonate and hydroxylamine. The cells were plated to select for reversal of auxotrophy due to a trpA(GAA211) mutation. With either mutagen, greater than 85% of the prototrophs obtained were due to suppressors of the trpA mutation. Approximately 12% of the ethyl methane sulfonate-induced and 37% of the hydroxylamine-induced suppressors were shown to be about 25% cotranscucible with metB, as is glyT. The transfer ribonucleic acid from four metB-linked suppressor strains (two from each mutagen) was examined by reversed-phase column (RPC-5) chromatography. In all four cases, the glycyl-transfer ribonucleic acid profile displayed an alteration of glyT transfer ribonucleic acid. All four suppressors responded to GAG in addition to GAA but did not suppress the known mutant codons of several other trpA mutants. Other properties are discussed, along with possible reasons for our success in obtaining these suppressors.
机译：在本文中，我们描述了成功分离glyT衍生的GAA抑制剂的方法。含有glyV55（一种用于GGA / G读数的基因），甲烷磺酸盐和羟胺的glyT +菌株。将细胞铺板以选择由于trpA（GAA211）突变而引起的营养缺陷型逆转。无论使用哪种诱变剂，所获得的原养型营养菌中有85％以上是由于trpA突变的抑制因子引起的。与glyT一样，约12％的由甲磺酸乙酯诱导的抑制剂和37％的由羟胺诱导的抑制剂与metB可共转化为约25％。通过反相色谱柱（RPC-5）色谱法检查了来自四个metB连接的抑制菌株（每个诱变剂两个）的转移核糖核酸。在所有四种情况下，甘氨酰转移核糖核酸的分布均显示出glyT转移核糖核酸的变化。除GAA之外，所有四个抑制子均对GAG作出反应，但没有抑制其他几个trpA突变体的已知突变密码子。讨论了其他性能，以及我们成功获得这些抑制剂的可能原因。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号