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首页> 外文期刊>Journal of bacteriology >Use of 8-hydroxyquinoline to enrich for temperature-sensitive mutants of Tetrahymena.
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Use of 8-hydroxyquinoline to enrich for temperature-sensitive mutants of Tetrahymena.

机译：使用8-羟基喹啉来丰富四膜虫对温度敏感的突变体。

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摘要

The effects of the chelating agent 8-hydroxyquinoline (Hq) on Tetrahymena thermophila were examined. Cell division was completely inhibited by 5 micrograms of Hq per ml. At this concentration deoxyribonucleic acid, ribonucleic acid, and protein syntheses were also completely and nonselectively inhibited. The inhibition was reversible after 6 h of Hq treatment. At concentrations above 20 micrograms/ml a 10,000-fold decrease in survival as seen after 2 h in the drug. The sensitivity of Tetrahymena to Hq was found to be dependent upon cell concentration, wild-type strain, medium, and length of time the culture is at 38 degrees C before Hq is added. Mutants of Tetrahymena that are unable to divide at the restrictive temperature, but which continue macromolecular synthesis, were found to be resistant to Hq treatment. Conditions were obtained in which more than a 1,000-fold difference in survival was seen between this class of mutant and the wild type. The effect of Hq on three other classes of temperature-sensitive mutants was examined, and the results are discussed.
机译：检验了螯合剂8-羟基喹啉（Hq）对嗜热四膜菌的影响。每毫升5微克Hq完全抑制细胞分裂。在该浓度下，脱氧核糖核酸，核糖核酸和蛋白质合成也被完全和非选择性地抑制。 Hq处理6小时后，抑制作用是可逆的。浓度超过20微克/毫升时，在药物中2小时后，存活率降低了10,000倍。发现四膜虫对Hq的敏感性取决于细胞浓度，野生型菌株，培养基以及添加Hq之前培养物在38摄氏度下的时间长度。发现在极限温度下不能分裂，但继续进行大分子合成的四膜虫突变体对Hq处理具有抗性。获得了这样的条件，其中在这类突变体和野生型之间观察到生存差异超过1,000倍。考察了Hq对其他三类温度敏感突变体的影响，并讨论了结果。

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