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首页> 外文期刊>Journal of bacteriology >Cloning, nucleotide sequence, and regulation of the Bacillus subtilis gpr gene, which codes for the protease that initiates degradation of small, acid-soluble proteins during spore germination.
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Cloning, nucleotide sequence, and regulation of the Bacillus subtilis gpr gene, which codes for the protease that initiates degradation of small, acid-soluble proteins during spore germination.

机译：枯草芽孢杆菌gpr基因的克隆，核苷酸序列和调控，该基因编码在孢子萌发过程中引发小酸溶性蛋白质降解的蛋白酶。

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摘要

The gpr gene, which codes for the protease that initiates degradation of small, acid-soluble proteins during spore germination, has been cloned from Bacillus megaterium and Bacillus subtilis, and its nucleotide sequence has been determined. Use of a translational gpr-lacZ fusion showed that the B. subtilis gpr gene was expressed primarily, if not exclusively, in the forespore compartment of the sporulating cell, with expression taking place approximately 1 h before expression of glucose dehydrogenase and ssp genes. gpr-lacZ expression was abolished in spoIIAC (sigF) and spoIIIE mutants but was reduced only approximately 50% in a spoIIIG (sigG) mutant. However, the kinetics of the initial approximately 50% of gpr-lacZ expression were unaltered in a spoIIIG mutant. The in vivo transcription start site of gpr has been identified and found to be identical to the in vitro start site on this gene with either E sigma F or E sigma G. Induction of sigma G synthesis in vivo turned on gpr-lacZ expression in parallel with synthesis of glucose dehydrogenase. These data are consistent with gpr transcription during sporulation first by E sigma F and then by E sigma G.
机译：已经从巨大芽孢杆菌和枯草芽孢杆菌中克隆了gpr基因，该基因编码在孢子萌发过程中引发小的酸溶性蛋白降解的蛋白酶。使用翻译的gpr-lacZ融合蛋白显示，枯草芽孢杆菌gpr基因主要（如果不是唯一的话）在孢子形成细胞的前孢子室中表达，表达发生在葡萄糖脱氢酶和ssp基因表达之前约1小时。 gpr-lacZ表达在spoIIAC（sigF）和spoIIIE突变体中被取消，但在spoIIIG（sigG）突变体中仅降低了约50％。但是，最初的约50％gpr-lacZ表达的动力学在spoIIIG突变体中没有改变。已鉴定出gpr的体内转录起始位点，并发现与该基因的体外起始位点具有E sigma F或E sigma G相同。体内对sigma G的诱导可并行开启gpr-lacZ表达与葡萄糖脱氢酶的合成。这些数据与孢子形成过程中首先由E sigma F然后是E sigma G的gpr转录一致。

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