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首页> 外文期刊>Journal of bacteriology >Actinomycin synthesis in Streptomyces antibioticus: enzymatic conversion of 3-hydroxyanthranilic acid to 4-methyl-3-hydroxyanthranilic acid.
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Actinomycin synthesis in Streptomyces antibioticus: enzymatic conversion of 3-hydroxyanthranilic acid to 4-methyl-3-hydroxyanthranilic acid.

机译：抗生素链霉菌中放线菌素的合成：3-羟基邻氨基苯甲酸向4-甲基-3-羟基邻氨基苯甲酸的酶促转化。

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A methyltransferase which utilizes 3-hydroxyanthranilic acid (HAA) as a substrate was identified in detergent-treated extracts of the bacterium Streptomyces antibioticus. The enzyme catalyzes the transfer of methyl groups from [14C]S-adenosylmethionine to HAA, but does not catalyze the methylation of 3-hydroxy-DL-kynurenine. Enzyme, substrate, time, and pH dependencies for the methyl transfer reaction were examined. Reaction products obtained from scaled-up reaction mixtures were fractionated by chromatography on Dowex 1, and the Dowex 1 fractions were examined by paper and thin-layer chromatography. One Dowex fraction was shown to contain a radioactive product with the chromatographic properties of 4-methyl-3-hydroxyanthranilic acid (MHA), a known intermediate in the biosynthesis of actinomycin. Available evidence indicates that the conversion of HAA to MHA is an early step in the biosynthesis of actinomycin by S. antibioticus and other actinomycin-producing streptomycetes.
机译：在细菌链霉菌的去污剂处理过的提取物中鉴定出了以3-羟基邻氨基苯甲酸（HAA）为底物的甲基转移酶。该酶催化甲基从[14C] S-腺苷甲硫氨酸向HAA的转移，但不催化3-羟基-DL-犬尿氨酸的甲基化。检查了甲基转移反应的酶，底物，时间和pH依赖性。由放大的反应混合物获得的反应产物通过在Dowex 1上的色谱法进行分级分离，并通过纸和薄层色谱法检查Dowex 1的级分。显示一个Dowex馏分包含具有4-甲基-3-羟基邻氨基苯甲酸（MHA）的色谱特性的放射性产物，该产物是放线菌素生物合成中的已知中间体。现有证据表明，HAA向MHA的转化是抗生素链球菌和其他产生放线菌的链霉菌生物合成放线菌的第一步。

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