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首页> 外文期刊>Journal of bacteriology >A new NAD+-dependent opine dehydrogenase from Arthrobacter sp. strain 1C.
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A new NAD+-dependent opine dehydrogenase from Arthrobacter sp. strain 1C.

机译：关节杆菌属一种新的NAD +依赖性阿片脱氢酶。菌株1C。

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摘要

A new NAD+-dependent opine dehydrogenase was purified to homogeneity from Arthrobacter sp. strain 1C isolated from soil by an enrichment culture technique. The enzyme has a molecular weight of about 70,000 and consists of two identical subunits with molecular weights of about 36,000. The enzyme catalyzed a reversible oxidation-reduction reaction of opine-type secondary amine dicarboxylic acids. In the oxidative deamination reaction, the enzyme was active toward unusual opines, such as N-[1-R-(carboxyl)ethyl]-S-methionine and N-[1-R-(carboxyl)ethyl]-S-phenylalanine. In the reductive secondary amine-forming reaction with NADH as a cofactor, the enzyme utilized L-amino acids such as L-methionine, L-isoleucine, L-valine, L-phenylalanine, L-leucine, L-alanine, and L-threonine as amino donors and alpha-keto acids such as pyruvate, oxaloacetate, glyoxylate, and alpha-ketobutyrate as amino acceptors. The product enzymatically synthesized from L-phenylalanine and pyruvate in the presence of NADH was identified as N-[1-R-(carboxyl)ethyl]-S-phenylalanine.
机译：从节杆菌中纯化出一种新的NAD +依赖性阿片脱氢酶使其同质。通过富集培养技术从土壤中分离出的1C菌株。该酶的分子量约为70,000，由两个相同的分子量约为36,000的亚基组成。该酶催化阿片型仲胺二羧酸的可逆氧化还原反应。在氧化脱氨反应中，该酶对不常见的阿片类诸如N- [1-R-（羧基）乙基] -S-甲硫氨酸和N- [1-R-（羧基）乙基] -S-苯丙氨酸具有活性。在以NADH为辅因子的还原性仲胺形成反应中，该酶利用了L-氨基酸，例如L-蛋氨酸，L-异亮氨酸，L-缬氨酸，L-苯丙氨酸，L-亮氨酸，L-丙氨酸和L-氨基酸。苏氨酸作为氨基供体，α-酮酸（例如丙酮酸，草酰乙酸，乙醛酸酯和α-酮丁酸酯）作为氨基受体。在NADH存在下由L-苯丙氨酸和丙酮酸酶促合成的产物被鉴定为N- [1-R-（羧基）乙基] -S-苯丙氨酸。

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