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首页> 外文期刊>Journal of bacteriology >Lincomycin increases synthetic rate and periplasmic pool size for cholera toxin.
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Lincomycin increases synthetic rate and periplasmic pool size for cholera toxin.

机译：林可霉素可增加霍乱毒素的合成速率和周质池大小。

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Increased enterotoxigenicity of Vibrio cholerae 569B grown with low concentrations of lincomycin, previously described in terms of increased extracellular biological activity (capillary permeability factor and fluid accumulation in ligated rabbit ileal loops), was further characterized. Polyacrylamide gel electrophoresis and single radial immunodiffusion showed that lincomycin-stimulated cells produced increased molar quantities of cholera toxin (CT) both extra- and intracellularly. The intracellular CT was released in comparable amounts by sonication, deoxycholate extraction, and polymyxin B treatment. Polymyxin B release of CT was nearly complete under conditions wherein only 6% of total cellular beta-galactosidase was released, implying a periplasmic pool of CT in stimulated cells. No intracellular choleragenoid (CT subunit B) was found in stimulated cells by polymyxin B release. No proteolysis of 14C-labeled CT was detected after prolonged incubation with sonicated nonstimulated cultures or sonicated concentrated cells. These data support the conclusion that the stimulatory effect of lincomycin involves an increase in the rate of synthesis of the CT molecule, and argue against alternative models involving inhibition of putative normal degradation of CT, increased release of otherwise cell-bound CT, or activation of inactive, or less active, forms of CT.
机译：用高浓度的林可霉素生长的霍乱弧菌569B的肠毒力增加，以前曾以增加的细胞外生物活性（毛细血管通透性因子和结扎的兔回肠环中的液体积聚）进行了描述。聚丙烯酰胺凝胶电泳和单次径向免疫扩散显示，受林可霉素刺激的细胞在细胞外和细胞内产生的摩尔霍乱毒素（CT）摩尔量均增加。通过超声处理，脱氧胆酸盐提取和多粘菌素B处理可释放相当数量的细胞内CT。在仅释放总细胞β-半乳糖苷酶总量6％的条件下，CT的多粘菌素B释放几乎完成，这意味着受刺激细胞中CT的胞质池。在多粘菌素B释放的刺激细胞中未发现细胞内类霍乱激素（CT亚基B）。与超声处理的非刺激培养物或超声处理的浓缩细胞长时间孵育后，未检测到14C标记的CT发生蛋白水解。这些数据支持这样的结论，即林可霉素的刺激作用涉及CT分子合成速率的增加，并且与其他模型相反，这些模型涉及抑制CT的假定正常降解，增加细胞结合CT的释放或激活CD。不活跃或不太活跃的CT形式。

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