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首页> 外文期刊>Journal of bacteriology >Molecular characterization of a fimbrial adhesin, F1845, mediating diffuse adherence of diarrhea-associated Escherichia coli to HEp-2 cells.
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Molecular characterization of a fimbrial adhesin, F1845, mediating diffuse adherence of diarrhea-associated Escherichia coli to HEp-2 cells.

机译：纤维粘附素F1845的分子表征，介导腹泻相关大肠杆菌对HEp-2细胞的弥漫性粘附。

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摘要

A fimbrial adhesin, designated F1845, was found to be responsible for the diffuse HEp-2 cell adherence of a diarrheal Escherichia coli isolate. The genetic determinant of F1845 was cloned, and the order of the genes necessary for production of F1845 was determined by maxicell analysis. Five polypeptides with apparent sizes of 10, 95, 27, 15.5, and 14.3 kilodaltons (kDa) were found to be encoded in that order by the F1845 determinant. The nucleotide sequence of the 14.3-kDa subunit gene was determined and found to share extensive homology in its signal sequence with the gene encoding the structural subunit of the AFA-1 hemagglutinin of a uropathogenic E. coli strain (A. Labigne-Roussel, M.A. Schmidt, W. Walz, and S. Falkow, J. Bacteriol. 162:1285-1292, 1985) but not in the region encoding the mature protein. Southern blot hybridizations indicated that the F1845 determinants are of chromosomal origin. Hybridization studies using a probe from the region encoding the 95-kDa polypeptide indicated that related sequences may be plasmid associated in some strains and chromosomal in others. Additional hybridization studies of E. coli isolates possessing sequence homology to the F1845 determinant suggest that the sequences in the 5' region of the F1845 structural subunit gene are more highly conserved than sequences in the 3' region.
机译：发现一种名为F1845的纤维粘附素与腹泻性大肠杆菌分离株的弥漫性HEp-2细胞粘附有关。克隆了F1845的遗传决定因素，并通过maxicell分析确定了生产F1845所需的基因顺序。发现F1845决定簇按此顺序编码了5个表观大小分别为10、95、27、15.5和14.3千道尔顿（kDa）的多肽。确定了14.3kDa亚基基因的核苷酸序列，并发现其信号序列与编码尿路致病性大肠杆菌菌株AFA-1血凝素结构亚基的基因（A.Labigne-Roussel，MA）具有广泛的同源性Schmidt，W.Walz和S.Falkow，J.Bacteriol.162：1285-1292，1985），但不在编码成熟蛋白质的区域中。 Southern印迹杂交表明，F1845决定簇是染色体起源的。使用来自编码95-kDa多肽的区域的探针进行的杂交研究表明，相关序列可能在某些菌株中与质粒相关，而在另一些菌株中与染色体相关。具有与F1845决定簇具有序列同源性的大肠杆菌分离株的其他杂交研究表明，与3'区域的序列相比，F1845结构亚基基因的5'区域的保守性更高。

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