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首页> 外文期刊>Journal of bacteriology >Anaerobic regulation of the hydrogenase 1 (hya) operon of Escherichia coli.
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Anaerobic regulation of the hydrogenase 1 (hya) operon of Escherichia coli.

机译：大肠杆菌氢化酶1（hya）操纵子的厌氧调节。

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摘要

Using a transcriptional fusion to the lacZ gene, we have analyzed the anaerobic regulation of the hydrogenase 1 (hya) operon in response to different anaerobic growth conditions and to mutations in regulatory genes. We found that the transcription of the hya operon was induced when the growth condition was changed from aerobic to anaerobic and that this induction was independent of Fnr but dependent on regulators AppY and ArcA. Furthermore, we found that the transcription of the hya operon was not regulated by the cyclic AMP-cyclic AMP receptor protein complex. Investigation of the effects of different anaerobic growth conditions on the expression of the hya operon showed that expression was induced by formate and repressed by nitrate. Formate induction was not mediated by the fhlA gene product, and nitrate repression was not mediated by the narL gene product. We found a high level of anaerobic expression of the hya operon in glucose medium supplemented with formate and in glycerol medium supplemented with fumarate, suggesting that hydrogenase isoenzyme 1 has a function during both fermentative growth and anaerobic respiration.
机译：使用对lacZ基因的转录融合，我们已经分析了氢化酶1（hya）操纵子的厌氧调节，以响应不同的厌氧生长条件和调节基因的突变。我们发现，当生长条件从有氧变为厌氧时，hya操纵子的转录被诱导，并且这种诱导独立于Fnr，但依赖于调节剂AppY和ArcA。此外，我们发现，hya操纵子的转录不受环状AMP-环状AMP受体蛋白复合物的调节。对不同厌氧生长条件对hya操纵子表达的影响的研究表明，该表达被甲酸盐诱导并被硝酸盐抑制。甲酰胺的诱导不是由fhlA基因产物介导的，硝酸盐阻抑不是由narL基因产物介导的。我们发现，在添加甲酸的葡萄糖培养基和添加富马酸的甘油培养基中，hya操纵子的厌氧表达水平很高，表明氢化酶同工酶1在发酵生长和厌氧呼吸过程中均具有功能。

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