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首页> 外文期刊>Journal of bacteriology >Construction of a chromosome map for the phage group II Staphylococcus aureus Ps55.
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Construction of a chromosome map for the phage group II Staphylococcus aureus Ps55.

机译：噬菌体组金黄色葡萄球菌Ps55的染色体图的构建。

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The genome size and a partial physical and genetic map have been defined for the phage group II Staphylococcus aureus Ps55. The genome size was estimated to be 2,771 kb by pulsed-field gel electrophoresis (PFGE) using the restriction enzymes SmaI, CspI, and SgrAI. The Ps55 chromosome map was constructed by transduction of auxotrophic and cryptic transposon insertions, with known genetic and physical locations in S. aureus NCTC 8325, into the Ps55 background. PFGE and DNA hybridization analysis were used to detect the location of the transposon in Ps55. Ps55 restriction fragments were then ordered on the basis of genetic conservation between the two strains. Cloned DNA probes containing the lactose operon (lac) and genes encoding staphylococcal protein A (spa), gamma hemolysin (hlg), and coagulase (coa) were also located on the map by PFGE and hybridization analysis. This methodology enabled a direct comparison of chromosomal organization between NCTC 8325 and Ps55 strains. The chromosome size, gene order, and some of the restriction sites are conserved between the two phage group strains.
机译：已经确定了噬菌体组II金黄色葡萄球菌Ps55的基因组大小以及部分物理和遗传图谱。使用限制酶SmaI，CspI和SgrAI通过脉冲场凝胶电泳（PFGE）估计基因组大小为2,771 kb。 Ps55染色体图谱是通过将营养缺陷型和隐性转座子插入物（在金黄色葡萄球菌NCTC 8325中具有已知的遗传和物理位置）转导到Ps55背景中而构建的。 PFGE和DNA杂交分析用于检测转座子在Ps55中的位置。然后根据两个菌株之间的遗传保守性对Ps55限制性片段进行排序。通过PFGE和杂交分析，还将含有乳糖操纵子（lac）和编码葡萄球菌蛋白A（spa），γ溶血素（hlg）和凝固酶（coa）的基因的克隆DNA探针定位在地图上。这种方法可以直接比较NCTC 8325和Ps55菌株之间的染色体组织。在两个噬菌体组菌株之间，染色体大小，基因顺序和一些限制性位点是保守的。

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