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首页> 外文期刊>Journal of bacteriology >Salmonella typhimurium mutants with reduced levels of transfer ribonucleic acid-inhibitable endodeoxyribonucleolytic activity.
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Salmonella typhimurium mutants with reduced levels of transfer ribonucleic acid-inhibitable endodeoxyribonucleolytic activity.

机译：鼠伤寒沙门氏菌突变体具有降低的转移核糖核酸抑制性内脱氧核糖核糖核酸分解活性的水平。

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摘要

Two methods of mutagenesis, chemical alkylation and insertion of the tetracycline resistance transposon Tn10, were used to generate mutants of Salmonella typhimurium which had reduced levels of endodeoxyribonucleolytic activity. The chemically induced mutations defined a locus, endA, which was cotransduced with serA at a low frequency and with metK at a high frequency. Three-factor crosses revealed that metK was between serA and endA. The major endodeoxyribonucleolytic activity in crude extracts of s. typhimurium was similar to the activity of Escherichia coli endonuclease I. A Tn10 insertion mutation of endA resulted in the most severe loss of endodeoxyribonucleolytic activity among the endA alleles studied. Two of the chemically induced mutations resulted in activities which were more thermolabile than the wild-type activity.
机译：诱变的两种方法，化学烷基化和四环素抗性转座子Tn10的插入，被用来产生鼠伤寒沙门氏菌的突变体，其具有降低的内脱氧核糖核酸活性。化学诱导的突变定义了一个基因座endA，它与serA低频共转导，与metK高频共转导。三因素杂交显示，metK位于serA和endA之间。 s的粗提物中主要的内氧核糖核酸分解活性。鼠伤寒沙门氏菌与大肠杆菌核酸内切酶I的活性相似。endA的Tn10插入突变导致研究的endA等位基因中最严重的内脱氧核糖核酸水解活性丧失。化学诱导的突变中的两个导致的活性比野生型活性更不耐热。

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