The Lactobacillus acidophilus S-layer protein gene expression site comprises two consensus promoter sequences, one of which directs transcription of stable mRNA.

H J Boot; C P Kolen; F J Andreadaki; R J Leer; P H Pouwels

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The Lactobacillus acidophilus S-layer protein gene expression site comprises two consensus promoter sequences, one of which directs transcription of stable mRNA.

机译：嗜酸乳杆菌S层蛋白基因表达位点包含两个共有启动子序列，其中一个指导稳定mRNA的转录。

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S-proteins are proteins which form a regular structure (S-layer) on the outside of the cell walls of many bacteria. Two S-protein-encoding genes are located in opposite directions on a 6.0-kb segment of the chromosome of Lactobacillus acidophilus ATCC 4356 bacteria. Inversion of this chromosomal segment occurs through recombination between two regions with identical sequences, thereby interchanging the expressed and the silent genes. In this study, we show that the region involved in recombination also has a function in efficient S-protein production. Two promoter sequences are present in the S-protein gene expression site, although only the most downstream promoter (P-1) is used to direct mRNA synthesis. S-protein mRNA directed by this promoter has a half-life of 15 min. Its untranslated leader can form a stable secondary structure in which the 5' end is base paired, whereas the ribosome-binding site is exposed. Truncation of this leader sequence results in a reduction in protein production, as shown by reporter gene analysis of Lactobacillus casei. The results obtained indicate that the untranslated leader sequence of S-protein mRNA is involved in efficient S-protein production.

机译：S蛋白是在许多细菌的细胞壁外部形成规则结构（S层）的蛋白。两个编码S蛋白的基因位于嗜酸乳杆菌ATCC 4356细菌的染色体的6.0 kb片段上，方向相反。该染色体区段的倒转是通过具有相同序列的两个区域之间的重组发生的，从而互换了表达的基因和沉默的基因。在这项研究中，我们表明参与重组的区域在高效S蛋白生产中也具有功能。尽管仅使用最下游的启动子（P-1）指导mRNA合成，但S蛋白基因表达位点中存在两个启动子序列。该启动子指导的S蛋白mRNA的半衰期为15分钟。它的非翻译前导序列可以形成稳定的二级结构，其中5'端是碱基对，而核糖体结合位点则暴露在外。如干酪乳杆菌的报道基因分析所示，该前导序列的截短导致蛋白质产生的减少。获得的结果表明，S蛋白mRNA的未翻译的前导序列与有效的S蛋白产生有关。

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《Journal of bacteriology》 |1996年第18期|共7页
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H J Boot; C P Kolen; F J Andreadaki; R J Leer; P H Pouwels;
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中图分类微生物学;
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1. Identification, cloning, and nucleotide sequence of a silent S-layer protein gene of Lactobacillus acidophilus ATCC 4356 which has extensive similarity with the S-layer protein gene of this species. [J] . H J Boot, C P Kolen, P H Pouwels Journal of bacteriology . 1995,第24期

机译：嗜酸乳杆菌ATCC 4356的沉默S层蛋白基因的鉴定，克隆和核苷酸序列，与该物种的S层蛋白基因具有广泛相似性。
2. S-layer protein of Lactobacillus acidophilus ATCC 4356: purification, expression in Escherichia coli, and nucleotide sequence of the corresponding gene. [J] . H J Boot, C P Kolen, J M van Noort, Journal of bacteriology . 1993,第19期

机译：嗜酸乳杆菌ATCC 4356的S层蛋白：纯化，在大肠杆菌中表达以及相应基因的核苷酸序列。
3. Activation Control of pur Gene Expression inLactococcus lactis: Proposal for a Consensus Activator Binding Sequence Based on Deletion Analysis and Site-Directed Mutagenesis of purC and purD Promoter Regions [J] . Mogens Kilstrup, Stine G. Jessing, Stephanie B. Wichmand-J?rgensen, Journal of bacteriology . 1998,第15期

机译：pur基因在乳酸乳球菌中的表达的激活控制：基于purC和purD启动子区域的缺失分析和定点诱变的共识激活子结合序列的建议。
4. Molecular Cloning and Heterologous Expression of linoleic acid isomerase gene from Lactobacillus reuteri and Lactobacillus acidophilus [C] . Xue Luo, LanWei Zhang, TieNan Luo, International Conference on Chemical Engineering and Advanced Materials . 2012

机译：来自乳杆菌和乳杆菌的亚油酸异构酶基因的分子克隆与异源表达
5. Heterodimeric deoxyguanosine/deoxyadenosine kinase from Lactobacillus acidophilus R-26: Affinity protein purification, molecular cloning, sequence of the genes, and expression in Escherichia coli. [D] . Ma, Grace Tak-Yi. 1993

机译：嗜酸乳杆菌R-26的异二聚体脱氧鸟苷/脱氧腺苷激酶：亲和蛋白纯化，分子克隆，基因序列和在大肠杆菌中的表达。
6. The Lactobacillus acidophilus S-layer protein gene expression site comprises two consensus promoter sequences one of which directs transcription of stable mRNA. [O] . H J Boot, C P Kolen, F J Andreadaki, 1996

机译：嗜酸乳杆菌S层蛋白基因表达位点包含两个共有启动子序列其中一个指导稳定mRNA的转录。
7. The Lactobacillus acidophilus S-layer protein gene expression site comprises two consensus promoter sequences, one of which directs transcription of stable mRNA. [O] . Boot, H J, Kolen, C P, Andreadaki, F J, 1996

机译：嗜酸乳杆菌S层蛋白基因表达位点包含两个共有启动子序列，其中一个指导稳定mRNA的转录。

The Lactobacillus acidophilus S-layer protein gene expression site comprises two consensus promoter sequences, one of which directs transcription of stable mRNA.

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