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首页> 外文期刊>Journal of bacteriology >Characterization of the aegA locus of Escherichia coli: control of gene expression in response to anaerobiosis and nitrate.
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Characterization of the aegA locus of Escherichia coli: control of gene expression in response to anaerobiosis and nitrate.

机译：大肠杆菌aegA基因座的表征：响应厌氧菌和硝酸盐而控制基因表达。

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Analysis of the DNA sequence upstream of the narQ gene, which encodes the second nitrate-responsive sensor-transmitter protein in Escherichia coli, revealed an open reading frame (ORF) whose product shows a high degree of similarity to a number of iron-sulfur proteins as well as to the beta subunit of glutamate synthase (gltD) of E. coli. This ORF, located at 53.0 min on the E. coli chromosome, is divergently transcribed and is separated by 206 bp from the narQ gene. Because of the small size of the intergenic region, we reasoned that the genes may be of related function and/or regulated in a similar fashion. An aegA-lacZ gene fusion was constructed and examined in vivo; aegA expression was induced 11-fold by anaerobiosis and repressed 5-fold by nitrate. This control was mediated by the fnr, narX, narQ, and narL gene products. Analysis of an aegA mutant indicated that the aegA gene product is not essential for cell respiration or fermentation or for the utilization of ammonium or the amino acids L-alanine, L-arginine, L-glutamic acid, glycine, and DL-serine as sole nitrogen sources. The ORF was designated aegA to reflect that it is an anaerobically expressed gene. The structural properties of the predicted AegA amino acid sequence and the regulation of aegA are discussed with regard to the possible function of aegA in E. coli.
机译：分析narQ基因上游的DNA序列，该序列编码大肠杆菌中的第二个硝酸盐反应性传感器-递质，揭示了一个开放阅读框（ORF），其产物与多种铁硫蛋白高度相似以及大肠杆菌的谷氨酸合酶（gltD）的β亚基。该ORF位于大肠杆菌染色体上的53.0分钟处，是发散转录的，与narQ基因相距206 bp。由于基因间区域的大小很小，我们认为这些基因可能具有相关功能和/或以相似的方式受到调控。构建了aegA-lacZ基因融合体并在体内进行了检查;厌氧菌诱导aegA表达11倍，硝酸盐抑制5倍。这种控制由fnr，narX，narQ和narL基因产物介导。对aegA突变体的分析表明，aegA基因产物对于细胞呼吸或发酵或仅利用铵或氨基酸L-丙氨酸，L-精氨酸，L-谷氨酸，甘氨酸和DL-丝氨酸不是必需的氮源。 ORF被命名为aegA，以反映它是一个厌氧表达的基因。关于aegA在大肠杆菌中的可能功能，讨论了预测的AegA氨基酸序列的结构特性和aegA的调控。

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