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首页> 外文期刊>Journal of bacteriology >Recombination-Promoting Activity of the Bacteriophage λ Rap Protein in Escherichia coli K-12
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Recombination-Promoting Activity of the Bacteriophage λ Rap Protein in Escherichia coli K-12

机译：噬菌体λRap蛋白在大肠杆菌K-12中的重组促进活性。

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The rap gene of bacteriophage λ was placed in the chromosome of an Escherichia coli K-12 strain in which the recBCD gene cluster had previously been replaced by the λ red genes and in which the recG gene had been deleted. Recombination between linear double-stranded DNA molecules and the chromosome was tested in variants of the recGΔ red+ rap+ strain bearing mutations in genes known to affect recombination in other cellular pathways. The linear DNA was a 4-kb fragment containing the cat gene, with flanking lac sequences, released from an infecting phage chromosome by restriction enzyme cleavage in the cell. Replacement of wild-type lacZ with lacZ::cat was monitored by measuring the production of Lac-deficient chloramphenicol-resistant bacterial progeny. The results of these experiments indicated that the λ rap gene could functionally substitute for the E. coli ruvC gene in Red-mediated recombination.
机译：噬菌体λ的 rap 基因被置于大肠杆菌 K-12菌株的染色体中，该菌株先前已替换了 recBCD 基因簇通过λ red 基因被删除，其中 recG 基因被删除。在 recG Δ red + rap 的变体中测试了线性双链DNA分子与染色体之间的重组 + 菌株在已知会影响其他细胞途径重组的基因中带有突变。线性DNA是一个4kb的片段，含有 cat 基因，带有侧翼的 lac 序列，通过限制酶在细胞中的裂解而从感染噬菌体染色体中释放出来。通过测量缺乏Lac的耐氯霉素的细菌后代的产量来监测用 lacZ :: cat 替代野生型 lacZ 。这些实验结果表明，λ rap 基因可以在功能上替代 E。介导的ruvC 基因在Red介导的重组中的表达

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