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首页> 外文期刊>Journal of bacteriology >Site-Specific Integrative Elements of Rhizobiophage 16-3 Can Integrate into Proline tRNA (CGG) Genes in Different Bacterial Genera
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Site-Specific Integrative Elements of Rhizobiophage 16-3 Can Integrate into Proline tRNA (CGG) Genes in Different Bacterial Genera

机译：根瘤菌噬菌体16-3的特定于位点的整合元件可以整合到不同细菌属的脯氨酸tRNA（CGG）基因中。

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摘要

The integrase protein of the Rhizobium meliloti 41 phage 16-3 has been classified as a member of the Int family of tyrosine recombinases. The site-specific recombination system of the phage belongs to the group in which the target site of integration (attB) is within a tRNA gene. Since tRNA genes are conserved, we expected that the target sequence of the site-specific recombination system of the 16-3 phage could occur in other species and integration could take place if the required putative host factors were also provided by the targeted cells. Here we report that a plasmid (pSEM167) carrying the attP element and the integrase gene (int) of the phage can integrate into the chromosomes of R. meliloti 1021 and eight other species. In all cases integration occurred at so-far-unidentified, putative proline tRNA (CGG) genes, indicating the possibility of their common origin. Multiple alignment of the sequences suggested that the location of the att core was different from that expected previously. The minimal attB was identified as a 23-bp sequence corresponding to the anticodon arm of the tRNA.
机译：苜蓿根瘤菌 41噬菌体 16-3 的整合酶蛋白已被归类为酪氨酸重组酶Int家族的成员。噬菌体的位点特异性重组系统属于其中整合的目标位点（ attB ）在tRNA基因内的组。由于tRNA基因是保守的，我们预期 16-3 噬菌体的位点特异性重组系统的目标序列可能会在其他物种中发生，如果所需的推定宿主因子也能发生整合，由目标细胞提供。在这里，我们报道一个带有 attP 元件和噬菌体整合酶基因（ int ）的质粒（pSEM167）可以整合到 R的染色体中。 meliloti 1021和其他八个物种。在所有情况下，整合都发生在迄今尚未确定的推定脯氨酸tRNA（CGG）基因上，表明它们可能共同起源。序列的多重比对表明 att 核心的位置与先前预期的不同。最小的 attB 被确定为对应于tRNA反密码子臂的23 bp序列。

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