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首页> 外文期刊>Journal of bacteriology >Immunolocalization of Hsp60 inLegionella pneumophila
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Immunolocalization of Hsp60 inLegionella pneumophila

机译：Hsp60在肺炎军团菌中的免疫定位

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One of the most abundant proteins synthesized by Legionella pneumophila, particularly during growth in a variety of eukaryotic host cells, is Hsp60, a member of the GroEL family of molecular chaperones. The present study was initiated in response to a growing number of reports suggesting that for some bacteria, includingL. pneumophila, Hsp60 may exist in extracytoplasmic locations. Immunolocalization techniques with Hsp60-specific monoclonal and polyclonal antibodies were used to define the subcellular location and distribution of Hsp60 in L. pneumophila grown in vitro, or in vivo inside of HeLa cells. For comparative purposesEscherichia coli, expressing recombinant L. pneumophila Hsp60, was employed. In contrast to E. coli, where Hsp60 was localized exclusively in the cytoplasm, inL. pneumophila Hsp60 was predominantly associated with the cell envelope, conforming to a distribution pattern typical of surface molecules that included the major outer membrane protein OmpS and lipopolysaccharide. Interestingly, heat-shocked L. pneumophila organisms exhibited decreased overall levels of cell-associated Hsp60 epitopes and increased relative levels of surface epitopes, suggesting that Hsp60 was released by stressed bacteria. Putative secretion of Hsp60 by L. pneumophila was further indicated by the accumulation of Hsp60 in the endosomal space, between replicating intracellular bacteria. These results are consistent with an extracytoplasmic location for Hsp60 in L. pneumophilaand further suggest both the existence of a novel secretion mechanism (not present in E. coli) and a potential role in pathogenesis.
机译：嗜肺军团菌（Legionella pneumophila）合成的最丰富的蛋白质之一，尤其是在各种真核宿主细胞中生长期间，是分子伴侣分子GroEL家族的成员Hsp60。响应于越来越多的报告表明对于某些细菌，包括 L，提出了本研究。肺炎，Hsp60可能存在于胞浆外。使用Hsp60特异性单克隆抗体和多克隆抗体的免疫定位技术来确定Hsp60在 L中的亚细胞定位和分布。在HeLa细胞内部或体内生长的肺炎为了比较目的，表达重组大肠杆菌的大肠杆菌。使用嗜肺细胞Hsp60。与 E相反。大肠杆菌，其中Hsp60仅位于 L细胞质中。肺炎热菌Hsp60主要与细胞包膜相关，符合包括主要外膜蛋白OmpS和脂多糖在内的表面分子的典型分布模式。有趣的是，热冲击的 L。嗜肺菌生物表现出与细胞相关的Hsp60表位的总体水平降低和表面表位的相对水平升高，这表明Hsp60是由压力细菌释放的。推定 L分泌Hsp60。 Hsp60在复制细胞内细菌之间的内体空间中的积累进一步表明了肺炎。这些结果与 L中Hsp60的胞外位置一致。肺炎并进一步暗示了新型分泌机制的存在（大肠杆菌中不存在）以及在发病机理中的潜在作用。

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