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首页> 外文期刊>Journal of bacteriology >The pcd Gene Encoding Piperideine-6-Carboxylate Dehydrogenase Involved in Biosynthesis of α-Aminoadipic Acid Is Located in the Cephamycin Cluster ofStreptomyces clavuligerus
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The pcd Gene Encoding Piperideine-6-Carboxylate Dehydrogenase Involved in Biosynthesis of α-Aminoadipic Acid Is Located in the Cephamycin Cluster ofStreptomyces clavuligerus

机译:参与生物合成α-氨基己二酸的编码哌啶子基-6-羧酸脱氢酶的pcd基因位于链霉菌的头霉素簇中

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摘要

Three open reading frames (ORFs) have been located downstream ofcefE in the cephamycin C gene cluster of Streptomyces clavuligerus. ORF13 (pcd) encodes a 496-amino-acid protein (molecular weight [MW], 52,488) with an N-terminal amino acid sequence identical to that of pure piperideine-6-carboxylate dehydrogenase. ORF14 (cmcT) encodes a 523-amino-acid protein (MW, 54,232) analogous to Streptomyces proteins for efflux and resistance to antibiotics. ORF15 (pbp74) encodes a high molecular weight penicillin-binding protein (MW, 74,094).
机译:三个开放阅读框(ORF)位于 Streptomyces clavuligerus 的头霉素C基因簇的 cefE 的下游。 ORF13( pcd )编码一个496个氨基酸的蛋白质(分子量[MW],52,488),其N末端氨基酸序列与纯哌啶-6-羧酸盐脱氢酶相同。 ORF14( cmcT )编码与链霉菌(Streptomyces)蛋白相似的523个氨基酸的蛋白质(MW,54,232),具有外排作用和对抗生素的抗性。 ORF15( pbp74 )编码一种高分子量青霉素结合蛋白(分子量,74,094)。

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