...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of bacteriology >Oligoribonuclease Is Encoded by a Highly Conserved Gene in the 3′-5′ Exonuclease Superfamily
【24h】

Oligoribonuclease Is Encoded by a Highly Conserved Gene in the 3′-5′ Exonuclease Superfamily

机译：寡核糖核酸酶由3'-5'核酸外切酶超家族中的高度保守的基因编码

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Oligoribonuclease, a 3′-to-5′ exoribonuclease specific for small oligoribonucleotides, was purified to homogeneity from extracts ofEscherichia coli. The purified protein is an α2 dimer of 40 kDa. NH2-terminal sequence analysis of the protein identified the gene encoding oligoribonuclease as yjeR(o204a), a previously reported open reading frame located at 94 min on the E. coli chromosome. However, as a consequence of the sequence information, the translation start site of this open reading frame has been revised. Cloning of yjeRled to overexpression of oligoribonuclease activity, and interruption of the cloned gene with a kanamycin resistance cassette eliminated the overexpression. On the basis of these data, we propose thatyjeR be renamed orn. Orthologs of oligoribonuclease are present in a wide range of organisms, extending up to humans.
机译：寡核糖核酸酶是一种特异于小寡核糖核苷酸的3'至5'核糖核酸外切酶，可从大肠杆菌的提取物中纯化至同质。纯化的蛋白质是40 kDa的α2二聚体。该蛋白质的NH 2 末端序列分析确定了编码寡核糖核酸酶的基因为 yjeR （ o204a ），这是先前报道的位于94位的开放阅读框在 E上的分钟。大肠染色体。然而，由于序列信息，该开放阅读框的翻译起始位点已被修改。克隆 yjeR 导致寡核糖核酸酶活性的过表达，并且用卡那霉素抗性盒中断克隆的基因消除了过表达。根据这些数据，我们建议将 yjeR 重命名为 orn​​ 。寡核糖核酸酶的直系同源物存在于广泛的生物中，一直延伸到人类。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号