...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of bacteriology >RpiR Homologues May Link Staphylococcus aureus RNAIII Synthesis and Pentose Phosphate Pathway Regulation
【24h】

RpiR Homologues May Link Staphylococcus aureus RNAIII Synthesis and Pentose Phosphate Pathway Regulation

机译：RpiR同源物可能链接金黄色葡萄球菌RNAIII合成和戊糖磷酸途径的调控。

获取原文
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Staphylococcus aureus is a medically important pathogen that synthesizes a wide range of virulence determinants. The synthesis of many staphylococcal virulence determinants is regulated in part by stress-induced changes in the activity of the tricarboxylic acid (TCA) cycle. One metabolic change associated with TCA cycle stress is an increased concentration of ribose, leading us to hypothesize that a pentose phosphate pathway (PPP)-responsive regulator mediates some of the TCA cycle-dependent regulatory effects. Using bioinformatics, we identified three potential ribose-responsive regulators that belong to the RpiR family of transcriptional regulators. To determine whether these RpiR homologues affect PPP activity and virulence determinant synthesis, the rpiR homologues were inactivated, and the effects on PPP activity and virulence factor synthesis were assessed. Two of the three homologues (RpiRB and RpiRC) positively influence the transcription of the PPP genes rpiA and zwf, while the third homologue (RpiRA) is slightly antagonistic to the other homologues. In addition, inactivation of RpiRC altered the temporal transcription of RNAIII, the effector molecule of the agr quorum-sensing system. These data confirm the close linkage of central metabolism and virulence determinant synthesis, and they establish a metabolic override for quorum-sensing-dependent regulation of RNAIII transcription.
机译：金黄色葡萄球菌是一种医学上重要的病原体，可合成多种毒力决定因素。许多葡萄球菌毒力决定因素的合成部分受应激诱导的三羧酸（TCA）循环活性变化的调节。与TCA周期应激相关的一种代谢变化是核糖浓度升高，这使我们推测，戊糖磷酸途径（PPP）响应调节剂介导了一些TCA周期依赖性调节作用。使用生物信息学，我们确定了三个潜在的核糖反应性调控子，它们属于转录调控子RpiR家族。为了确定这些RpiR同源物是否影响PPP活性和毒力决定因素的合成，将 rpiR 同源物失活，并评估其对PPP活性和毒力因子合成的影响。三个同源物中的两个（RpiRB和RpiRC）对PPP基因 rpiA 和 zwf 的转录产生正向影响，而第三个同源物（RpiRA）则与其他同源物略有对立。另外，RpiRC的失活改变了RNAIII的瞬时转录，RNAIII是 agr 群体感应系统的效应分子。这些数据证实了中央代谢和毒力决定因素合成的紧密联系，并且它们为RNA感应转录的群体感应依赖性调节建立了代谢替代。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号