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首页> 外文期刊>Journal of bacteriology >Regulation of the Transposase of Tn4652by the Transposon-Encoded Protein TnpC
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Regulation of the Transposase of Tn4652by the Transposon-Encoded Protein TnpC

机译：转座子编码蛋白TnpC对Tn4652转座酶的调控

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Transposition is a DNA reorganization reaction potentially deleterious for the host. The frequency of transposition is limited by the amount of transposase. Therefore, strict regulation of a transposase is required to keep control over the destructive multiplication of the mobile element. We have shown previously that the expression of the transposase (tnpA) of thePseudomonas putida PaW85 transposon Tn4652 is positively affected by integration host factor. Here, we present evidence that the amount of the transposase of Tn4652 inP. putida cells is controlled by the transposon-encoded protein (TnpC). Sequence analysis of the 120-amino-acid-long TnpC, coded just downstream of the tnpA gene, showed that it has remarkable similarity to the putative polypeptide encoded by the mercury resistance transposon Tn5041. As determined by quantitative Western blot analysis, the abundance of TnpA was reduced up to 10-fold in the intact tnpC background. In vivo experiments using transcriptional and translational fusions of thetnpA gene and the reporter gene gusA indicated that TnpC operates in the regulation of the transposase of Tn4652 at the post-transcriptional level.
机译：转座是对宿主潜在有害的DNA重组反应。转座的频率受转座酶量的限制。因此，需要严格调节转座酶以保持对可移动元件的破坏性繁殖的控制。先前我们已经表明，整合宿主因子对恶臭假单胞菌PaW85转座子Tn 4652 的转座酶（ tnpA ）的表达产生正向影响。在这里，我们提供证据表明在 P中Tn 4652 的转座酶数量。 putida 细胞受转座子编码蛋白（TnpC）的控制。编码 tnpA 基因下游的120个氨基酸长的TnpC的序列分析表明，它与抗汞转座子Tn 5041 < / em>。通过定量蛋白质印迹分析确定，在完整的 tnpC 背景下，TnpA的丰度降低了10倍。使用 tnpA 基因和报告基因 gusA 的转录和翻译融合物进行的体内实验表明，TnpC在调节Tn 4652 在转录后级别。

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