Genome Plasticity among RelatedLactococcus Strains: Identification of Genetic Events Associated with Macrorestriction Polymorphisms

Pascal Le Bourgeois; Marie-Line Daveran-Mingot; Paul Ritzenthaler

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Genome Plasticity among RelatedLactococcus Strains: Identification of Genetic Events Associated with Macrorestriction Polymorphisms

机译：相关乳球菌菌株之间的基因组可塑性：鉴定与宏观限制性多态性相关的遗传事件。

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The genomic diversity of nine strains of the Lactococcus lactis subsp. cremoris (NCDO712, NCDO505, NCDO2031, NCDO763, MMS36, C2, LM0230, LM2301, and MG1363) was studied by macrorestriction enzyme analysis using pulsed-field gel electrophoresis. These strains were considered adequate for the investigation of genomic plasticity because they have been described as belonging to the same genetic lineage. Comparison of ApaI and SmaI genome fingerprints of each strain revealed the presence of several macrorestriction fragment length polymorphisms (RFLPs), despite a high degree of similarity of the generated restriction patterns. The physical map of the MG1363 chromosome was used to establish a genome map of the other strains and allocate the RFLPs to five regions. Southern hybridization analysis correlated the polymorphic regions with genetic events such as chromosomal inversion, integration of prophage DNA, and location of the transposon-like structures carrying conjugative factor or oligopeptide transport system.

机译：9株乳酸乳球菌亚种的基因组多样性。使用脉冲场凝胶电泳通过宏观限制性酶分析法研究了 cremoris （NCDO712，NCDO505，NCDO2031，NCDO763，MMS36，C2，LM0230，LM2301和MG1363）。这些菌株被认为足以研究基因组可塑性，因为它们被描述为属于同一遗传谱系。比较每个菌株的 Apa I和 Sma I基因组指纹图谱，尽管所产生的限制性酶切图谱高度相似，但仍存在几种宏限制性片段长度多态性（RFLP）。。 MG1363染色体的物理图谱用于建立其他菌株的基因组图谱，并将RFLP分配到五个区域。 Southern杂交分析将多态性区域与遗传事件相关，例如染色体倒置，噬菌体DNA整合以及带有结合因子或寡肽转运系统的转座子样结构的位置。 展开▼

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《Journal of bacteriology》 |2000年第9期|共11页

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Pascal Le Bourgeois; Marie-Line Daveran-Mingot; Paul Ritzenthaler;
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中图分类微生物学;

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6. Genome Plasticity among Related Lactococcus Strains: Identification of Genetic Events Associated with Macrorestriction Polymorphisms [O] . Pascal Le Bourgeois, Marie-Line Daveran-Mingot, Paul Ritzenthaler 2000

机译：相关乳球菌菌株之间的基因组可塑性：识别与宏观限制性多态性相关的遗传事件。

7. Genome Plasticity among Related Lactococcus Strains: Identification of Genetic Events Associated with Macrorestriction Polymorphisms [O] . Pascal Le Bourgeois, Marie-Line Daveran-Mingot, Paul Ritzenthaler 2000

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机译：基于 u043e u0434 u043d u043e u043d u0443 u043a u043b u043b u0435 u043e u0442 u0438 u0434 u043d u043e u0433 u043e多态性的遗传识别方法u043e u043b u0438 u0433 u043e u043d u0443 u043a u043b u0435 u043e u0442 u0438 u0434 u043d u043d u043e u0433 u043e u0431 u0438 u043e u043b u043e u0433 u043e u0447 u0435 u0441 u043a u043e世纪的（ u0431 u0438 u043e u0447 u0438 u043f u0430）

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5. Genome-wide scanning of genetic polymorphisms [P] . 外国专利： US2004110166A1 . 2004-06-10

机译：全基因组遗传多态性扫描

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Genome Plasticity among RelatedLactococcus Strains: Identification of Genetic Events Associated with Macrorestriction Polymorphisms

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