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首页> 外文期刊>Journal of bacteriology >Osa Protein Constitutes a Strong Oncogenic Suppression System That Can Block vir-Dependent Transfer of IncQ Plasmids between Agrobacterium Cells and the Establishment of IncQ Plasmids in Plant Cells
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Osa Protein Constitutes a Strong Oncogenic Suppression System That Can Block vir-Dependent Transfer of IncQ Plasmids between Agrobacterium Cells and the Establishment of IncQ Plasmids in Plant Cells

机译：Osa蛋白构成了一个强大的致癌抑制系统，该系统可以阻止农杆菌细胞之间vir-IncQ质粒在病毒中的转移以及植物细胞中IncQ质粒的建立。

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The osa (oncogenic suppressive activity) gene of the IncW group plasmid pSa is sufficient to suppress tumorigenesis by Agrobacterium tumefaciens. osa confers oncogenic suppression by inhibiting VirE2 protein export. This result is similar, but not identical, to that of oncogenic suppression by the IncQ plasmid RSF1010. We conducted a series of experiments to compare oncogenic suppression by these two systems. Agrobacterium strains harboring plasmids containing osa are more able to effect oncogenic suppression than are similar strains containing various RSF1010 derivatives. When osa is present within a donor Agrobacterium strain that also carries a derivative of RSF1010, the transfer of RSF1010 derivatives to recipient bacteria and their establishment in plants are blocked. Oncogenic suppression is still effected when the osa gene is integrated into the Agrobacterium chromosome, suggesting that it is the osa gene product that is active in suppression and that suppression does not require a protein-nucleic acid intermediate like that described for IncQ plasmids. Extracellular complementation experiments with tobacco leaf disks indicated that Osa blocks stable transfer of RSF1010 to plant cells by inhibiting transfer of VirE2, which is essential for the transfer of RSF1010 into plant cells, and not by inhibiting the actual transfer of RSF1010 itself. Our results suggest that Osa and RSF1010 cause oncogenic suppression by using different mechanisms.
机译：IncW组质粒pSa的 osa （致癌抑制活性）基因足以抑制根癌农杆菌的致癌作用。 osa 通过抑制VirE2蛋白输出而赋予了致癌作用。该结果与IncQ质粒RSF1010的致癌抑制作用相似但不相同。我们进行了一系列实验，比较了这两种系统的致癌作用。含有含 osa 质粒的农杆菌菌株比含有各种RSF1010衍生物的类似菌株更能实现致癌作用。当供体 Agrobacterium 菌株中还含有RSF1010衍生物时，出现了 osa ，RSF1010衍生物向受体细菌的转移及其在植物中的建立就被阻止了。当 osa 基因整合到农杆菌染色体中时，致癌抑制仍然有效，这表明抑制作用有效的是 osa 基因产物并且该抑制不需要像IncQ质粒中所述的蛋白质-核酸中间体。烟草叶圆盘的细胞外互补实验表明，Osa通过抑制VirE2的转移来阻止RSF1010向植物细胞的稳定转移，这对于RSF1010向植物细胞的转移至关重要，而不是通过抑制RSF1010本身的实际转移。我们的结果表明，Osa和RSF1010通过使用不同的机制导致了致癌性抑制。

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