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首页> 美国政府科技报告 >Analysis of proteins essential for Agrobacterium mediated DNA transfer to plant cells. (Progress report).
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Analysis of proteins essential for Agrobacterium mediated DNA transfer to plant cells. (Progress report).

机译：分析农杆菌介导的DNa转移到植物细胞必需的蛋白质。 （进度报告）。

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The tumor inducing (Ti) plasmid of Agrobacterium contains two regions important for infection and transformation of plant cells. One region, the T-DNA, is transferred as a single strand into the plant cell, while the virulence (vir) region is responsible for recognition of susceptible cells, synthesis of the T-DNA single strand (T-strand), formation of a T-strand protein complex and transfer of this complex into susceptible cells. A DNA binding protein, VirE2, was identified as a product of the vir region. Sequencing of the 9000 kilobase pair virB region has been completed. Expression of 10 of the predicted 11 open reading frames (ORFs) was demonstrated in Escherichia coli. Translational coupling was demonstrated for 5 ORFs. Hydropathy analysis indicates that 9 of 11 ORFs have hydrophobic regions that could permit membrane channel formation. In related work, analysis of protons that potentiate movement of plant viruses was discussed, with indications that the tobacco mosaic virus (TMV) protein P30 may mediate transfer of TMV RNA through plasmadesmata. Also, using the T-DNA element as a marker, genes responsible for abnormal flower development are being cloned and isolated. 3 refs. (MHB)

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