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首页> 外文期刊>Journal of bacteriology >Global Analyses of Transcriptomes and Proteomes of a Parent Strain and an l-Threonine-Overproducing Mutant Strain
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Global Analyses of Transcriptomes and Proteomes of a Parent Strain and an l-Threonine-Overproducing Mutant Strain

机译：亲本菌株和过量生产l-苏氨酸的突变株的转录组和蛋白质组的全局分析

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摘要

We compared the transcriptome, proteome, and nucleotide sequences between the parent strain Escherichia coli W3110 and the l-threonine-overproducing mutant E. coli TF5015. DNA macroarrays were used to measure mRNA levels for all of the genes of E. coli, and two-dimensional gel electrophoresis was used to compare protein levels. It was observed that only 54 of 4,290 genes (1.3%) exhibited differential expression profiles. Typically, genes such as aceA, aceB, icdA, gltA, glnA, leu operon, proA, thrA, thrC, and yigJ, which are involved in the glyoxylate shunt, the tricarboxylic acid cycle, and amino acid biosynthesis (l-glutamine, l-leucine, proline, and l-threonine), were significantly upregulated, whereas the genes dadAX, hdeA, hdeB, ompF, oppA, oppB, oppF, yfiD, and many ribosomal protein genes were downregulated in TF5015 compared to W3110. The differential expression such as upregulation of thr operon and expression of yigJ would result in an accumulation of l-threonine in TF5015. Furthermore, two significant mutations, thrA345 and ilvA97, which are essential for overproduction of l-threonine, were identified in TF5015 by the sequence analysis. In particular, expression of the mutated thrABC (pATF92) in W3110 resulted in a significant incremental effect on l-threonine production. Upregulation of aceBA and downregulation of b1795, hdeAB, oppA, and yfiD seem to be linked to a low accumulation of acetate in TF5015. Such comprehensive analyses provide information regarding the regulatory mechanism of l-threonine production and the physiological consequences in the mutant stain.
机译：我们比较了亲本菌株大肠杆菌 W3110和过量生产l-苏氨酸的突变体 E 的转录组，蛋白质组和核苷酸序列。 大肠杆菌 TF5015。 DNA宏阵列用于测量 E 所有基因的mRNA水平。用大肠杆菌和二维凝胶电泳比较蛋白质水平。观察到4,290个基因中只有54个（1.3％）表现出差异表达谱。通常，诸如 aceA ， aceB ， icdA ， gltA ， glnA ， leu 操纵子， proA ， thrA ， thrC 和 yigJ 在乙醛酸分流器中，三羧酸循环和氨基酸生物合成（l-谷氨酰胺，l-亮氨酸，脯氨酸和l-苏氨酸）被显着上调，而基因 dadAX ， hdeA ， hdeB ， ompF ， oppA ， oppB ， oppF ， yfiD 和许多核糖体蛋白基因在TF5015中的表达均低于W3110。 thr 操纵子的上调和 yigJ 的表达差异会导致TF5015中L-苏氨酸的积累。此外，通过序列分析，在TF5015中鉴定了两个重要的突变， thrA345 和 ilvA97 ，它们是过量生产l-苏氨酸所必需的。特别是，W3110中突变的 thrABC （pATF92）的表达对L-苏氨酸的产生产生了明显的增量影响。 aceBA 的上调和b1795， hdeAB ， oppA 和 yfiD 的下调似乎与低积累有关TF5015中的乙酸盐。这样的综合分析提供了有关L-苏氨酸产生的调节机制和突变体染色中的生理后果的信息。

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