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首页> 外文期刊>Journal of bacteriology >Regulation of Sulfur Assimilation Pathways in Burkholderia cenocepacia: Identification of Transcription Factors CysB and SsuR and Their Role in Control of Target Genes
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Regulation of Sulfur Assimilation Pathways in Burkholderia cenocepacia: Identification of Transcription Factors CysB and SsuR and Their Role in Control of Target Genes

机译：伯克霍尔德酒菌中硫同化途径的调控：转录因子CysB和SsuR的鉴定及其在靶基因控制中的作用

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Two genes encoding transcriptional regulators involved in sulfur assimilation pathways in Burkholderia cenocepacia strain 715j have been identified and characterized functionally. Knockout mutations in each of the B. cenocepacia genes were constructed and introduced into the genome of 715j by allelic replacement. Studies on the utilization of various sulfur sources by 715j and the obtained mutants demonstrated that one of the B. cenocepacia regulators, designated CysB, is preferentially involved in the control of sulfate transport and reduction, while the other, designated SsuR, is required for aliphatic sulfonate utilization. Using transcriptional promoter-lacZ fusions and DNA-binding experiments, we identified several target promoters for positive control by CysB and/or SsuR—sbpp (preceding the sbp cysT cysW cysA ssuR cluster), cysIp (preceding the cysI cysD1 cysN cysH cysG cluster), cysD2p (preceding a separate cluster, cysD2 cysNC), and ssuDp (located upstream of the ssuDCB operon)—and we demonstrated overlapping functions of CysB and SsuR at particular promoters. We also demonstrated that the cysB gene is negatively controlled by both CysB and SsuR but the ssuR gene itself is not significantly regulated as a separate transcription unit. The function of B. cenocepacia CysB (in vivo and in vitro) appeared to be independent of the presence of acetylserine, the indispensable coinducer of the CysB regulators of Escherichia coli and Salmonella. The phylogenetic relationships among members of the “CysB family” in the γ and β subphyla are presented.
机译：已经鉴定并编码了 Burkholderia cenocepacia 菌株715j中参与硫同化途径的两个转录调控因子基因，并对其功能进行了表征。每个 B中的敲除突变。通过等位基因置换，构建了新洋葱基因，并将其引入715j基因组。对715j及其获得的突变体利用各种硫源的研究表明，其中的 B之一。命名为CysB的先验菌调节剂优先参与硫酸盐的运输和还原控制，而脂族磺酸盐的利用则需要另一个名为SsuR的调节剂。使用转录启动子- lacZ 融合蛋白和DNA结合实验，我们确定了一些目标启动子，可通过CysB和/或SsuR- sbpp （在 sbp cysT之前）进行阳性控制cysW cysA ssuR 群集）， cysIp （在 cysI cysD1 cysN cysH cysG 群集之前）， cysD2p （在单独的群集之前， cysD2 cysNC ）和 ssuDp （位于 ssuDCB 操纵子的上游），我们展示了CysB和SsuR在特定启动子上的重叠功能。我们还证明了 cysB 基因同时受到CysB和SsuR的负调控，但 ssuR 基因本身并未作为单独的转录单位受到显着调控。 B的功能。 cenocepacia CysB（体内和体外）似乎独立于乙酰丝氨酸的存在，乙酰丝氨酸是大肠杆菌和沙门氏菌的CysB调节剂必不可少的共同诱因。介绍了γ和β亚门的“ CysB家族”成员之间的系统发育关系。

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